Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23191 | 5' | -52.2 | NC_005259.1 | + | 45654 | 0.7 | 0.674654 |
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Target: 5'- cGGGCUCGaggcuGCCUGCGAGgccgccgaGGCAugGAu -3' miRNA: 3'- -CUCGAGCg----UGGACGCUUg-------UUGUugCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 51211 | 0.7 | 0.708714 |
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Target: 5'- cGAGCgugCGCACCgcgauagcgaggccgGUGAGCu-CAGCGAg -3' miRNA: 3'- -CUCGa--GCGUGGa--------------CGCUUGuuGUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 27242 | 0.69 | 0.729246 |
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Target: 5'- cGAcCUCGCAgCCUugcGCGAACAACAcCGGc -3' miRNA: 3'- -CUcGAGCGU-GGA---CGCUUGUUGUuGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 60804 | 0.69 | 0.760901 |
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Target: 5'- -cGUUuaCGCGCCcgaUGuCGGGCAACAGCGAg -3' miRNA: 3'- cuCGA--GCGUGG---AC-GCUUGUUGUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 25099 | 0.68 | 0.791338 |
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Target: 5'- -uGCcCGCGCCgGCugaucgauGGGCAGCGACGAc -3' miRNA: 3'- cuCGaGCGUGGaCG--------CUUGUUGUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 37831 | 0.68 | 0.791338 |
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Target: 5'- cGAGCUCGC-CCU-UGAGCcaguCGGCGAg -3' miRNA: 3'- -CUCGAGCGuGGAcGCUUGuu--GUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 2041 | 0.66 | 0.902074 |
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Target: 5'- cGAGCUguuucuugCGCGCCUcGCGGugAAUcGCGu -3' miRNA: 3'- -CUCGA--------GCGUGGA-CGCUugUUGuUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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