Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23191 | 5' | -52.2 | NC_005259.1 | + | 58564 | 0.67 | 0.847329 |
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Target: 5'- cGAGCUCGaCAUCgaGUGGcuCGACGGCGGu -3' miRNA: 3'- -CUCGAGC-GUGGa-CGCUu-GUUGUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 60804 | 0.69 | 0.760901 |
|
Target: 5'- -cGUUuaCGCGCCcgaUGuCGGGCAACAGCGAg -3' miRNA: 3'- cuCGA--GCGUGG---AC-GCUUGUUGUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 65431 | 0.66 | 0.895042 |
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Target: 5'- cGAGCUCGcCGCCUacaaGGACAuCGACa- -3' miRNA: 3'- -CUCGAGC-GUGGAcg--CUUGUuGUUGcu -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 65910 | 0.66 | 0.880166 |
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Target: 5'- aGAGC-CGCGCa-GCGAugAGCgGGCGGc -3' miRNA: 3'- -CUCGaGCGUGgaCGCUugUUG-UUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 66379 | 0.66 | 0.887737 |
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Target: 5'- uGGCacuacCGCACCUcGcCGAACAAgGGCGGu -3' miRNA: 3'- cUCGa----GCGUGGA-C-GCUUGUUgUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 67811 | 0.67 | 0.872332 |
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Target: 5'- uGAGC-CGCucACCUGCuacGACuGCAACGGc -3' miRNA: 3'- -CUCGaGCG--UGGACGc--UUGuUGUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 68041 | 0.72 | 0.552729 |
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Target: 5'- cAGCUCGCGCaacgGCucAACGGCGACGAg -3' miRNA: 3'- cUCGAGCGUGga--CGc-UUGUUGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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