Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23192 | 3' | -50.4 | NC_005259.1 | + | 67014 | 0.66 | 0.946014 |
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Target: 5'- aCGaGCGGUcGGAGUAcaacuacGCGGCCAU-CGGg -3' miRNA: 3'- -GCgCGUUA-CUUCAU-------CGUCGGUAuGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 33330 | 0.66 | 0.951132 |
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Target: 5'- aGCGCGGUGGuGGUgcacAGCGacGCCGUGCc- -3' miRNA: 3'- gCGCGUUACU-UCA----UCGU--CGGUAUGcc -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 118 | 0.66 | 0.951132 |
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Target: 5'- gCGCGcCGAUGAGGUAGaugaGGCgGUGguuugUGGu -3' miRNA: 3'- -GCGC-GUUACUUCAUCg---UCGgUAU-----GCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 19091 | 0.66 | 0.951132 |
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Target: 5'- cCGCGUAccGggGUGcCAGaCCGauuUGCGGa -3' miRNA: 3'- -GCGCGUuaCuuCAUcGUC-GGU---AUGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 1681 | 0.66 | 0.959583 |
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Target: 5'- uGCGCGGUGAucccuGGUAG-AGCgCGUACc- -3' miRNA: 3'- gCGCGUUACU-----UCAUCgUCG-GUAUGcc -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 58202 | 0.66 | 0.963406 |
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Target: 5'- aGCGCAAUGucGUAguccuugcgcacGguGCCG-AUGGa -3' miRNA: 3'- gCGCGUUACuuCAU------------CguCGGUaUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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