Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23192 | 3' | -50.4 | NC_005259.1 | + | 59875 | 0.67 | 0.941574 |
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Target: 5'- aCGCGCccc--GGUacgGGCAGCCGUGCu- -3' miRNA: 3'- -GCGCGuuacuUCA---UCGUCGGUAUGcc -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 60028 | 0.68 | 0.912689 |
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Target: 5'- cCGUGCcggGAcGUAGguGCCAgcggcgACGGc -3' miRNA: 3'- -GCGCGuuaCUuCAUCguCGGUa-----UGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 61774 | 0.69 | 0.876732 |
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Target: 5'- uGCGUGAUGAAcgacGGCAGCCGcgcCGGu -3' miRNA: 3'- gCGCGUUACUUca--UCGUCGGUau-GCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 64492 | 0.69 | 0.843227 |
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Target: 5'- cCGCGCcg-GGAGUGGC-GCUGcGCGGc -3' miRNA: 3'- -GCGCGuuaCUUCAUCGuCGGUaUGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 65906 | 0.77 | 0.41174 |
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Target: 5'- cCGCGCAgcgAUGAGcgGGCGGCCAUugcGCGGc -3' miRNA: 3'- -GCGCGU---UACUUcaUCGUCGGUA---UGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 67014 | 0.66 | 0.946014 |
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Target: 5'- aCGaGCGGUcGGAGUAcaacuacGCGGCCAU-CGGg -3' miRNA: 3'- -GCgCGUUA-CUUCAU-------CGUCGGUAuGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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