Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23192 | 3' | -50.4 | NC_005259.1 | + | 9585 | 0.74 | 0.623386 |
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Target: 5'- uCGCGC-AUGgcGUcGGCGGCCAUgaagaACGGc -3' miRNA: 3'- -GCGCGuUACuuCA-UCGUCGGUA-----UGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 6154 | 0.7 | 0.834253 |
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Target: 5'- gCGCGCGGuUGAcg-AGCAGCCGcuCGGc -3' miRNA: 3'- -GCGCGUU-ACUucaUCGUCGGUauGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 4525 | 1.13 | 0.002187 |
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Target: 5'- aCGCGCAAUGAAGUAGCAGCCAUACGGg -3' miRNA: 3'- -GCGCGUUACUUCAUCGUCGGUAUGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 1868 | 0.7 | 0.79526 |
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Target: 5'- uCGUGCGugagcaccgcuGUGAgccgcuugucgauGGUGGCGGCCAUGCc- -3' miRNA: 3'- -GCGCGU-----------UACU-------------UCAUCGUCGGUAUGcc -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 1681 | 0.66 | 0.959583 |
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Target: 5'- uGCGCGGUGAucccuGGUAG-AGCgCGUACc- -3' miRNA: 3'- gCGCGUUACU-----UCAUCgUCG-GUAUGcc -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 118 | 0.66 | 0.951132 |
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Target: 5'- gCGCGcCGAUGAGGUAGaugaGGCgGUGguuugUGGu -3' miRNA: 3'- -GCGC-GUUACUUCAUCg---UCGgUAU-----GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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