Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23192 | 3' | -50.4 | NC_005259.1 | + | 18582 | 0.7 | 0.834253 |
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Target: 5'- gGUGCcuUGAccGGcucgGGCAGCgGUGCGGg -3' miRNA: 3'- gCGCGuuACU--UCa---UCGUCGgUAUGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 6154 | 0.7 | 0.834253 |
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Target: 5'- gCGCGCGGuUGAcg-AGCAGCCGcuCGGc -3' miRNA: 3'- -GCGCGUU-ACUucaUCGUCGGUauGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 43011 | 0.69 | 0.843227 |
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Target: 5'- aGCGCcgaggugaacaAcgGAAGUAGCAGCgGgAUGGc -3' miRNA: 3'- gCGCG-----------UuaCUUCAUCGUCGgUaUGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 64492 | 0.69 | 0.843227 |
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Target: 5'- cCGCGCcg-GGAGUGGC-GCUGcGCGGc -3' miRNA: 3'- -GCGCGuuaCUUCAUCGuCGGUaUGCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 26460 | 0.69 | 0.868731 |
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Target: 5'- uGgGCAccGAGGUGGCGGgCAgcuCGGc -3' miRNA: 3'- gCgCGUuaCUUCAUCGUCgGUau-GCC- -5' |
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| 23192 | 3' | -50.4 | NC_005259.1 | + | 58202 | 0.66 | 0.963406 |
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Target: 5'- aGCGCAAUGucGUAguccuugcgcacGguGCCG-AUGGa -3' miRNA: 3'- gCGCGUUACuuCAU------------CguCGGUaUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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