Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23194 | 5' | -59.3 | NC_005259.1 | + | 65137 | 0.66 | 0.572828 |
Target: 5'- cGGCGGccacccccGACAagUCGAaacGGgCCAUGGCCc -3' miRNA: 3'- cCCGCU--------CUGU--AGCU---CCgGGUACCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 50860 | 0.66 | 0.572828 |
Target: 5'- -cGCGAGGCAgauugcaaUCGAuGCCgAUGGUCGg -3' miRNA: 3'- ccCGCUCUGU--------AGCUcCGGgUACCGGU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 45207 | 0.66 | 0.541773 |
Target: 5'- cGGGCGAGuucGCGUUGA-GCCCGauGCCc -3' miRNA: 3'- -CCCGCUC---UGUAGCUcCGGGUacCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 28009 | 0.66 | 0.541773 |
Target: 5'- gGGGCGGGGuCGcCGGGGUugcgcgCguUGGCCAg -3' miRNA: 3'- -CCCGCUCU-GUaGCUCCG------GguACCGGU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 27464 | 0.66 | 0.531542 |
Target: 5'- -cGCGGGAaAUUGGGGCCggggucggUGUGGCCAc -3' miRNA: 3'- ccCGCUCUgUAGCUCCGG--------GUACCGGU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 46598 | 0.67 | 0.511298 |
Target: 5'- aGGCGAGcagcGCGUCGuuggcGGUCUugGUGGCCu -3' miRNA: 3'- cCCGCUC----UGUAGCu----CCGGG--UACCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 28340 | 0.67 | 0.511298 |
Target: 5'- uGGCGGcGAaAUCGAGGCCaCGgugcUGGCUg -3' miRNA: 3'- cCCGCU-CUgUAGCUCCGG-GU----ACCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 21924 | 0.67 | 0.511298 |
Target: 5'- -cGCGAGGCAgUCGAGGCCaucgagucuCAcUGGCgCAa -3' miRNA: 3'- ccCGCUCUGU-AGCUCCGG---------GU-ACCG-GU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 47846 | 0.67 | 0.501297 |
Target: 5'- cGGuGCGAG-CuuuUCGAGcGCCUuguUGGCCu -3' miRNA: 3'- -CC-CGCUCuGu--AGCUC-CGGGu--ACCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 23286 | 0.67 | 0.501297 |
Target: 5'- cGGGCGcggugguuGGGUAUCGGGGUCCGgcgcGGUCGg -3' miRNA: 3'- -CCCGC--------UCUGUAGCUCCGGGUa---CCGGU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 17840 | 0.67 | 0.491381 |
Target: 5'- cGGGUGGGc---CGAGGCCacccCGUGGUCAg -3' miRNA: 3'- -CCCGCUCuguaGCUCCGG----GUACCGGU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 58332 | 0.67 | 0.481558 |
Target: 5'- cGGGCagcGAGAUcagGUCGGuGGCCUcgGGCa- -3' miRNA: 3'- -CCCG---CUCUG---UAGCU-CCGGGuaCCGgu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 60344 | 0.68 | 0.462205 |
Target: 5'- aGGCGGcGAUcagGUCGAGGCCCu--GCCc -3' miRNA: 3'- cCCGCU-CUG---UAGCUCCGGGuacCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 57938 | 0.68 | 0.462205 |
Target: 5'- -aGCGAGuugaGCAcCGAGG-CCAUGGCCc -3' miRNA: 3'- ccCGCUC----UGUaGCUCCgGGUACCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 61052 | 0.68 | 0.448904 |
Target: 5'- cGGGCGgcucaccuugugccGGGCAUCGGcauaggcgucgucGGCCUcgGGCUu -3' miRNA: 3'- -CCCGC--------------UCUGUAGCU-------------CCGGGuaCCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 14957 | 0.68 | 0.443269 |
Target: 5'- aGGGCG--GCAUgGAGGCgCAUucaaGGCCGc -3' miRNA: 3'- -CCCGCucUGUAgCUCCGgGUA----CCGGU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 10611 | 0.68 | 0.442333 |
Target: 5'- -aGCGAGcacacguguggguGCGUCGAGGCCgAacagcuUGGCCu -3' miRNA: 3'- ccCGCUC-------------UGUAGCUCCGGgU------ACCGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 58937 | 0.68 | 0.433966 |
Target: 5'- cGGGCGcgguGGCAgCGucGGCCU-UGGCCAg -3' miRNA: 3'- -CCCGCu---CUGUaGCu-CCGGGuACCGGU- -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 53016 | 0.68 | 0.433966 |
Target: 5'- uGGGCGcugcgguGGCGUCGAGGCaUCGUGaCCu -3' miRNA: 3'- -CCCGCu------CUGUAGCUCCG-GGUACcGGu -5' |
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23194 | 5' | -59.3 | NC_005259.1 | + | 51436 | 0.68 | 0.424777 |
Target: 5'- uGGGCGGGcCA-CGGGGCCUcgcGCCGa -3' miRNA: 3'- -CCCGCUCuGUaGCUCCGGGuacCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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