Results 1 - 20 of 55 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23200 | 5' | -54.1 | NC_005259.1 | + | 29902 | 0.66 | 0.854964 |
Target: 5'- aGCaCAAGGGguggcugcccgagacCAUCGACGcGUGgcCGCCa -3' miRNA: 3'- -UGaGUUCCU---------------GUGGCUGC-CACauGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 52334 | 0.66 | 0.851648 |
Target: 5'- gGCUC--GGACA-CGcCGGUGUGcCGCUg -3' miRNA: 3'- -UGAGuuCCUGUgGCuGCCACAU-GCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 19865 | 0.66 | 0.851648 |
Target: 5'- cGCUCGcgcccgccGGGAuCGCCGACGcc--GCGCCc -3' miRNA: 3'- -UGAGU--------UCCU-GUGGCUGCcacaUGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 19106 | 0.66 | 0.851648 |
Target: 5'- aACUCAccGGCGCUGgu-GUGUGCGUCg -3' miRNA: 3'- -UGAGUucCUGUGGCugcCACAUGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 60421 | 0.66 | 0.851648 |
Target: 5'- uGCUCgGGGGugGCCcACGGggcgGccACGCCc -3' miRNA: 3'- -UGAG-UUCCugUGGcUGCCa---Ca-UGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 38711 | 0.66 | 0.846608 |
Target: 5'- gACUguAGGuugaccaccgcugccGCcguACCGGCGGUGgccCGCCg -3' miRNA: 3'- -UGAguUCC---------------UG---UGGCUGCCACau-GCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 17881 | 0.66 | 0.842349 |
Target: 5'- cGCUCGguuGGACccccgcaccgcgcGCCGAUGcGUGUggAUGCCc -3' miRNA: 3'- -UGAGUu--CCUG-------------UGGCUGC-CACA--UGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 66840 | 0.66 | 0.833669 |
Target: 5'- gGCUCAugccgcccgcacGGGcCACCGAuCGGccuUGUucgcaccGCGCCa -3' miRNA: 3'- -UGAGU------------UCCuGUGGCU-GCC---ACA-------UGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 4151 | 0.66 | 0.825682 |
Target: 5'- gUUCAAGGACGCgauggucaCGAUGGUc-ACGCUg -3' miRNA: 3'- uGAGUUCCUGUG--------GCUGCCAcaUGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 36911 | 0.66 | 0.825682 |
Target: 5'- cACUgaCGGGGAUaucguGCCGACGGUGaguguUGCUg -3' miRNA: 3'- -UGA--GUUCCUG-----UGGCUGCCACau---GCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 63552 | 0.66 | 0.825682 |
Target: 5'- uGCUCGGcGGcCACCGacgcgacgaGCGGgauaUACGCCg -3' miRNA: 3'- -UGAGUU-CCuGUGGC---------UGCCac--AUGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 44966 | 0.67 | 0.807372 |
Target: 5'- -gUCAucGGGAUGCCGAgggcCGGUGcgACuGCCg -3' miRNA: 3'- ugAGU--UCCUGUGGCU----GCCACa-UG-CGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 30545 | 0.67 | 0.807372 |
Target: 5'- -aUCAuGGcCACCGAgCGGuUGUugACGCCc -3' miRNA: 3'- ugAGUuCCuGUGGCU-GCC-ACA--UGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 10446 | 0.67 | 0.807372 |
Target: 5'- -aUCGAGuGGCucgaCGGCGGUGUccuGCGCa -3' miRNA: 3'- ugAGUUC-CUGug--GCUGCCACA---UGCGg -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 43653 | 0.67 | 0.797945 |
Target: 5'- -aUCu---GCGCCGACGGUGUcguUGCCg -3' miRNA: 3'- ugAGuuccUGUGGCUGCCACAu--GCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 8488 | 0.67 | 0.788351 |
Target: 5'- cGCUguc-GGCACUGAUGGUGUGCaCCa -3' miRNA: 3'- -UGAguucCUGUGGCUGCCACAUGcGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 15987 | 0.67 | 0.788351 |
Target: 5'- -gUCGAGuGAuCACCGcCGGUGgcCGCa -3' miRNA: 3'- ugAGUUC-CU-GUGGCuGCCACauGCGg -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 30294 | 0.67 | 0.788351 |
Target: 5'- aGCUCuacGGugGCCcauuaGACGGcGaGCGCCg -3' miRNA: 3'- -UGAGuu-CCugUGG-----CUGCCaCaUGCGG- -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 62732 | 0.68 | 0.7687 |
Target: 5'- cGCUCGAccGgACCGGCGGcaGUACGCg -3' miRNA: 3'- -UGAGUUccUgUGGCUGCCa-CAUGCGg -5' |
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23200 | 5' | -54.1 | NC_005259.1 | + | 39601 | 0.68 | 0.758665 |
Target: 5'- -aUCGuguGGGuggugccauCAgCGACGGUGUACGCg -3' miRNA: 3'- ugAGU---UCCu--------GUgGCUGCCACAUGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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