Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23206 | 3' | -58.5 | NC_005259.1 | + | 35308 | 0.67 | 0.586085 |
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Target: 5'- aGAUGaccgagGGCGGUGUGU-ACGcGACCCc -3' miRNA: 3'- gCUACca----CCGCCACGCGcUGU-CUGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 19209 | 0.67 | 0.596531 |
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Target: 5'- -cGUGGUG-CGGgcgGCGaCGugGGACaCCg -3' miRNA: 3'- gcUACCACcGCCa--CGC-GCugUCUG-GG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 66660 | 0.66 | 0.607004 |
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Target: 5'- ---cGGUcGGCGGUGCuGCGAU--GCUCa -3' miRNA: 3'- gcuaCCA-CCGCCACG-CGCUGucUGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 10230 | 0.66 | 0.611199 |
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Target: 5'- aGAUGGcgcucacugagcacGGCGGUGUGCuugaugaGCGGGCCg -3' miRNA: 3'- gCUACCa-------------CCGCCACGCGc------UGUCUGGg -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 33879 | 0.66 | 0.616447 |
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Target: 5'- ---cGGUGagcacacGCGGgaaccacccGCGCGACGGGCCg -3' miRNA: 3'- gcuaCCAC-------CGCCa--------CGCGCUGUCUGGg -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 3101 | 0.66 | 0.632205 |
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Target: 5'- gCGAUGaGcgGGCGGccauUGCGCGGCAccgugggcagcggacGGCaCCg -3' miRNA: 3'- -GCUAC-Ca-CCGCC----ACGCGCUGU---------------CUG-GG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 43980 | 0.66 | 0.649012 |
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Target: 5'- cCGAg---GGCGGUGC-CGAUGGACgCu -3' miRNA: 3'- -GCUaccaCCGCCACGcGCUGUCUGgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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