Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23206 | 3' | -58.5 | NC_005259.1 | + | 67162 | 0.71 | 0.32728 |
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Target: 5'- uCGAUGGUGGCGGccaUGCcGCcGAUgauGCCCg -3' miRNA: 3'- -GCUACCACCGCC---ACG-CG-CUGuc-UGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 41633 | 0.72 | 0.319728 |
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Target: 5'- -cAUGGUGGCGGUGC-CGgucuGCGGGCgCu -3' miRNA: 3'- gcUACCACCGCCACGcGC----UGUCUGgG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 29963 | 0.72 | 0.312307 |
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Target: 5'- uGGUGGggcgGGCGGUGacCGUccaACGGACCCc -3' miRNA: 3'- gCUACCa---CCGCCAC--GCGc--UGUCUGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 35674 | 0.73 | 0.257643 |
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Target: 5'- ---cGGUGGUGGUGCacaGCGACGccguGCCCg -3' miRNA: 3'- gcuaCCACCGCCACG---CGCUGUc---UGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 51268 | 0.74 | 0.238662 |
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Target: 5'- gCGGUGGUcaCGGUGCGCGGCAaGcggcagcGCCCg -3' miRNA: 3'- -GCUACCAccGCCACGCGCUGU-C-------UGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 65875 | 0.77 | 0.146938 |
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Target: 5'- cCGGUGG-GGCGGUcaaucgGCGgGACGGuCCCg -3' miRNA: 3'- -GCUACCaCCGCCA------CGCgCUGUCuGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 57879 | 0.98 | 0.004591 |
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Target: 5'- uCGAU-GUGGCGGUGCGCGACAGACCCc -3' miRNA: 3'- -GCUAcCACCGCCACGCGCUGUCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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