Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23206 | 3' | -58.5 | NC_005259.1 | + | 67162 | 0.71 | 0.32728 |
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Target: 5'- uCGAUGGUGGCGGccaUGCcGCcGAUgauGCCCg -3' miRNA: 3'- -GCUACCACCGCC---ACG-CG-CUGuc-UGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 15568 | 0.71 | 0.342778 |
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Target: 5'- ---aGGUGGCcacccucgGGgccaGCGCGACGGGCCg -3' miRNA: 3'- gcuaCCACCG--------CCa---CGCGCUGUCUGGg -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 31526 | 0.69 | 0.427937 |
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Target: 5'- uGAUcGG-GGUGGUGcCGUGAUAGAUCg -3' miRNA: 3'- gCUA-CCaCCGCCAC-GCGCUGUCUGGg -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 41119 | 0.69 | 0.427937 |
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Target: 5'- aCGAUGucGGCGGUGCccGCGucgcgcagccuCAGGCCCc -3' miRNA: 3'- -GCUACcaCCGCCACG--CGCu----------GUCUGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 6384 | 0.69 | 0.440825 |
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Target: 5'- gCGAUGGUcaacgucauuuucgaGGCGGUGCcCuGAUGuGGCCCg -3' miRNA: 3'- -GCUACCA---------------CCGCCACGcG-CUGU-CUGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 26637 | 0.69 | 0.455821 |
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Target: 5'- uCGAUGGccUGGC-GUGCGCucGGCAGguucggcgcACCCg -3' miRNA: 3'- -GCUACC--ACCGcCACGCG--CUGUC---------UGGG- -5' |
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| 23206 | 3' | -58.5 | NC_005259.1 | + | 43980 | 0.66 | 0.649012 |
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Target: 5'- cCGAg---GGCGGUGC-CGAUGGACgCu -3' miRNA: 3'- -GCUaccaCCGCCACGcGCUGUCUGgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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