Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23221 | 3' | -58.1 | NC_005259.1 | + | 19202 | 0.72 | 0.290559 |
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Target: 5'- gCGAGCUCGU-GGUGcgggCGGCGaCGUGg -3' miRNA: 3'- aGCUCGAGCAgCCACa---GCCGUcGCAC- -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 10326 | 0.73 | 0.257175 |
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Target: 5'- aUCGGGCUCGgcgaCGGgcucggCGGCAGCGg- -3' miRNA: 3'- -AGCUCGAGCa---GCCaca---GCCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 27364 | 0.74 | 0.221332 |
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Target: 5'- aUCGGGCUuggugcccggcaCGgugaccggcggCGGUGUCGGCAGCGg- -3' miRNA: 3'- -AGCUCGA------------GCa----------GCCACAGCCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 56547 | 0.74 | 0.221332 |
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Target: 5'- cUCGGGCUCGUCGGUcUCGGguuCGGUGa- -3' miRNA: 3'- -AGCUCGAGCAGCCAcAGCC---GUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 58908 | 0.78 | 0.13629 |
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Target: 5'- cUCGGGCUCGUCGGcggucuUGUCgucggcgggcgcgguGGCAGCGUc -3' miRNA: 3'- -AGCUCGAGCAGCC------ACAG---------------CCGUCGCAc -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 53535 | 0.79 | 0.114748 |
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Target: 5'- aCGGGCUCGgugccCGGUGUCGGCucgGGCGg- -3' miRNA: 3'- aGCUCGAGCa----GCCACAGCCG---UCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 10947 | 0.82 | 0.070052 |
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Target: 5'- gUCGAcCUCGUCGGUGUCGGUcugaggGGUGUGg -3' miRNA: 3'- -AGCUcGAGCAGCCACAGCCG------UCGCAC- -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 52765 | 1.08 | 0.000891 |
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Target: 5'- aUCGAGCUCGUCGGUGUCGGCAGCGUGu -3' miRNA: 3'- -AGCUCGAGCAGCCACAGCCGUCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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