Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23221 | 3' | -58.1 | NC_005259.1 | + | 53605 | 0.66 | 0.640869 |
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Target: 5'- ---uGCUCGUCGGgGUCaGCAGgCGUc -3' miRNA: 3'- agcuCGAGCAGCCaCAGcCGUC-GCAc -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 56547 | 0.74 | 0.221332 |
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Target: 5'- cUCGGGCUCGUCGGUcUCGGguuCGGUGa- -3' miRNA: 3'- -AGCUCGAGCAGCCAcAGCC---GUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 57311 | 0.67 | 0.59859 |
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Target: 5'- aUCGAGCacCGgCGGUGUCGGU-GUGg- -3' miRNA: 3'- -AGCUCGa-GCaGCCACAGCCGuCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 58202 | 0.67 | 0.577576 |
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Target: 5'- gUGGGCUUGcCGGUGUCagGGuCAGUGg- -3' miRNA: 3'- aGCUCGAGCaGCCACAG--CC-GUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 58908 | 0.78 | 0.13629 |
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Target: 5'- cUCGGGCUCGUCGGcggucuUGUCgucggcgggcgcgguGGCAGCGUc -3' miRNA: 3'- -AGCUCGAGCAGCC------ACAG---------------CCGUCGCAc -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 58971 | 0.66 | 0.609141 |
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Target: 5'- gUCGggcAGCUCGUCGGcGaCGGCgAGCuUGg -3' miRNA: 3'- -AGC---UCGAGCAGCCaCaGCCG-UCGcAC- -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 59127 | 0.71 | 0.36725 |
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Target: 5'- aUCGAGCagagCGUCGagcUCGGCAGCGg- -3' miRNA: 3'- -AGCUCGa---GCAGCcacAGCCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 60284 | 0.67 | 0.587016 |
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Target: 5'- cCGGGCggccaucgUCGUCGGUG-CGGCcacggacGGCGa- -3' miRNA: 3'- aGCUCG--------AGCAGCCACaGCCG-------UCGCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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