Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23221 | 3' | -58.1 | NC_005259.1 | + | 50407 | 0.66 | 0.640869 |
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Target: 5'- cCGAGCgCGcCGGUGccuugaccggcUCgGGCAGCGg- -3' miRNA: 3'- aGCUCGaGCaGCCAC-----------AG-CCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 52630 | 0.66 | 0.640869 |
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Target: 5'- gUCGAGCUgCGUCGGcUGUgGGgcgaGGUGa- -3' miRNA: 3'- -AGCUCGA-GCAGCC-ACAgCCg---UCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 24249 | 0.66 | 0.640869 |
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Target: 5'- gCGAGUUCGgCGaGUG-CGuGCAcGCGUGg -3' miRNA: 3'- aGCUCGAGCaGC-CACaGC-CGU-CGCAC- -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 53605 | 0.66 | 0.640869 |
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Target: 5'- ---uGCUCGUCGGgGUCaGCAGgCGUc -3' miRNA: 3'- agcuCGAGCAGCCaCAGcCGUC-GCAc -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 37961 | 0.66 | 0.651443 |
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Target: 5'- uUCGGGCUC-UUGG--UCGGCAGUGc- -3' miRNA: 3'- -AGCUCGAGcAGCCacAGCCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 51631 | 0.66 | 0.651443 |
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Target: 5'- aCGAGgUCGUCGGag-CGGUAGgCGa- -3' miRNA: 3'- aGCUCgAGCAGCCacaGCCGUC-GCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 44162 | 0.66 | 0.662 |
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Target: 5'- gUCGAGCagcgCGcCGGUGUCGGagaaaAGuCGa- -3' miRNA: 3'- -AGCUCGa---GCaGCCACAGCCg----UC-GCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 11133 | 0.66 | 0.666215 |
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Target: 5'- gCGAGCcgUCGccugcggguaacacuUCGGUG-CGGCAGUGc- -3' miRNA: 3'- aGCUCG--AGC---------------AGCCACaGCCGUCGCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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