Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23221 | 3' | -58.1 | NC_005259.1 | + | 10326 | 0.73 | 0.257175 |
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Target: 5'- aUCGGGCUCGgcgaCGGgcucggCGGCAGCGg- -3' miRNA: 3'- -AGCUCGAGCa---GCCaca---GCCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 19202 | 0.72 | 0.290559 |
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Target: 5'- gCGAGCUCGU-GGUGcgggCGGCGaCGUGg -3' miRNA: 3'- aGCUCGAGCAgCCACa---GCCGUcGCAC- -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 44400 | 0.72 | 0.29763 |
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Target: 5'- cCGAGCaCGUCGGUGcccgccugacCGGCAGCGc- -3' miRNA: 3'- aGCUCGaGCAGCCACa---------GCCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 59127 | 0.71 | 0.36725 |
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Target: 5'- aUCGAGCagagCGUCGagcUCGGCAGCGg- -3' miRNA: 3'- -AGCUCGa---GCAGCcacAGCCGUCGCac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 10435 | 0.69 | 0.456763 |
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Target: 5'- gCGAGCUCGacaUCGaGUGgcucgaCGGCGGUGUc -3' miRNA: 3'- aGCUCGAGC---AGC-CACa-----GCCGUCGCAc -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 40140 | 0.69 | 0.485831 |
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Target: 5'- gCGAGCUCGccgucgguugUCGGUGcccgcccCGGCAGCu-- -3' miRNA: 3'- aGCUCGAGC----------AGCCACa------GCCGUCGcac -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 51346 | 0.68 | 0.525888 |
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Target: 5'- gUGAGCUCGgauaugccCGGUGgcacCGGCAcaccgcGCGUGu -3' miRNA: 3'- aGCUCGAGCa-------GCCACa---GCCGU------CGCAC- -5' |
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| 23221 | 3' | -58.1 | NC_005259.1 | + | 11133 | 0.66 | 0.666215 |
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Target: 5'- gCGAGCcgUCGccugcggguaacacuUCGGUG-CGGCAGUGc- -3' miRNA: 3'- aGCUCG--AGC---------------AGCCACaGCCGUCGCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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