Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23230 | 3' | -57.3 | NC_005259.1 | + | 59154 | 0.66 | 0.645548 |
Target: 5'- --gGUGCCGGGgAGCugcuCAGGGCgcuuauacaUGGCg -3' miRNA: 3'- uuaUACGGCCCgUCGc---GUUCUG---------GCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 32696 | 0.66 | 0.634691 |
Target: 5'- ----aGCCGGGCGGUgGUucGuacACUGGCa -3' miRNA: 3'- uuauaCGGCCCGUCG-CGuuC---UGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 55101 | 0.66 | 0.634691 |
Target: 5'- ---cUGCCGauGGCGGCGaaaaauGcACCGGCu -3' miRNA: 3'- uuauACGGC--CCGUCGCguu---C-UGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 52888 | 0.66 | 0.634691 |
Target: 5'- -----cUCGGGC-GCGCGAGuACgGGCa -3' miRNA: 3'- uuauacGGCCCGuCGCGUUC-UGgCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 68703 | 0.66 | 0.634691 |
Target: 5'- ----aGgCGGGCAGCGCG---UCGGUg -3' miRNA: 3'- uuauaCgGCCCGUCGCGUucuGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 14209 | 0.66 | 0.633605 |
Target: 5'- ----gGCCGGGaucaacuaccCGGCGCGcgucgugcguaucGGGCCGGg -3' miRNA: 3'- uuauaCGGCCC----------GUCGCGU-------------UCUGGCCg -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 44677 | 0.66 | 0.623831 |
Target: 5'- ----cGuuGGGCAGCGU--GGCCcGCa -3' miRNA: 3'- uuauaCggCCCGUCGCGuuCUGGcCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 68797 | 0.66 | 0.612978 |
Target: 5'- ----cGCCgGGGCGGUGUcGGugcCCGGUa -3' miRNA: 3'- uuauaCGG-CCCGUCGCGuUCu--GGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 34115 | 0.66 | 0.612978 |
Target: 5'- cGUGUcGCCGGGCGcgaGCAcGuCCGGUa -3' miRNA: 3'- uUAUA-CGGCCCGUcg-CGUuCuGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 46788 | 0.66 | 0.612978 |
Target: 5'- ----cGCCGGGCcgaacaccgAGCcuGCGAGcgcaccACCGGCc -3' miRNA: 3'- uuauaCGGCCCG---------UCG--CGUUC------UGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 53552 | 0.66 | 0.602141 |
Target: 5'- uGUcgGCuCGGGCGGUGCGAu-CCGaGUa -3' miRNA: 3'- uUAuaCG-GCCCGUCGCGUUcuGGC-CG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 9044 | 0.66 | 0.602141 |
Target: 5'- cAUGUGCgGGGCGGUa----ACCGGCu -3' miRNA: 3'- uUAUACGgCCCGUCGcguucUGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 50393 | 0.66 | 0.602141 |
Target: 5'- --cGUGUCGcGGCucgccgAGCGCGccggugccuuGACCGGCu -3' miRNA: 3'- uuaUACGGC-CCG------UCGCGUu---------CUGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 61595 | 0.66 | 0.601059 |
Target: 5'- cGGUggGCCggugGGGuCGGCGgGgaacagcAGACCGGCa -3' miRNA: 3'- -UUAuaCGG----CCC-GUCGCgU-------UCUGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 1233 | 0.67 | 0.59133 |
Target: 5'- ------aCGaGGaCGGCuGCAAGACCGGCc -3' miRNA: 3'- uuauacgGC-CC-GUCG-CGUUCUGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 36223 | 0.67 | 0.59133 |
Target: 5'- --gAUGCCGagcaccuuGGcCAGCGCGAGcGCCuGCg -3' miRNA: 3'- uuaUACGGC--------CC-GUCGCGUUC-UGGcCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 54517 | 0.67 | 0.59133 |
Target: 5'- --gAUGUgGGGUGGCGgguCGAGuauCCGGCc -3' miRNA: 3'- uuaUACGgCCCGUCGC---GUUCu--GGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 2011 | 0.67 | 0.59133 |
Target: 5'- ----gGCCaucGGGUGGCGC---ACCGGCa -3' miRNA: 3'- uuauaCGG---CCCGUCGCGuucUGGCCG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 17171 | 0.67 | 0.590251 |
Target: 5'- ----aGCUGacgacaaGGCGGCGCAGGaaGCCGcGCg -3' miRNA: 3'- uuauaCGGC-------CCGUCGCGUUC--UGGC-CG- -5' |
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23230 | 3' | -57.3 | NC_005259.1 | + | 64021 | 0.67 | 0.580552 |
Target: 5'- ----cGcCCGaGGC-GCgGCGAGGCCGGUg -3' miRNA: 3'- uuauaC-GGC-CCGuCG-CGUUCUGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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