Results 1 - 20 of 113 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23264 | 3' | -59 | NC_005259.1 | + | 54198 | 0.66 | 0.619376 |
Target: 5'- uCAGGCGgCGGC-CCUUgacgGGCuggauACCGGGCa -3' miRNA: 3'- -GUCUGUgGCUGcGGAG----CCG-----UGGCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 55603 | 0.66 | 0.619376 |
Target: 5'- -cGAUACC-ACGUCUCGcGCGCCuuGGCc -3' miRNA: 3'- guCUGUGGcUGCGGAGC-CGUGGc-UCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 19215 | 0.66 | 0.619376 |
Target: 5'- gCGGGCGgCGACGUg--GGaCACCGAGUc -3' miRNA: 3'- -GUCUGUgGCUGCGgagCC-GUGGCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 35794 | 0.66 | 0.618327 |
Target: 5'- -cGACGCCG-CGCCUCaacaggacGGUGCCGuacgaccccgcauAGCc -3' miRNA: 3'- guCUGUGGCuGCGGAG--------CCGUGGC-------------UCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 43015 | 0.66 | 0.608888 |
Target: 5'- gAGAacaaACCGGCgauGCCgccgagcaGGcCGCCGAGCa -3' miRNA: 3'- gUCUg---UGGCUG---CGGag------CC-GUGGCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 23454 | 0.66 | 0.60784 |
Target: 5'- gCAGAuugcuuuCGCaCGGgGCCUCGGCauugucgguGCCGAcGCc -3' miRNA: 3'- -GUCU-------GUG-GCUgCGGAGCCG---------UGGCU-CG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 51506 | 0.66 | 0.60784 |
Target: 5'- -cGACGCCGccgaucucgacgcGCGugaCCUCGuGCACgGGGCc -3' miRNA: 3'- guCUGUGGC-------------UGC---GGAGC-CGUGgCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 15062 | 0.66 | 0.598418 |
Target: 5'- -cGAUACCGugGCCacccUGGC-CCuuGAGCc -3' miRNA: 3'- guCUGUGGCugCGGa---GCCGuGG--CUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 35994 | 0.66 | 0.598418 |
Target: 5'- -cGACGgCGAgGCCggaCuGCACCGAGg -3' miRNA: 3'- guCUGUgGCUgCGGa--GcCGUGGCUCg -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 30133 | 0.66 | 0.598418 |
Target: 5'- -cGGCugCGAUaCCUCgGGCagcucgACCGGGCc -3' miRNA: 3'- guCUGugGCUGcGGAG-CCG------UGGCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 13010 | 0.66 | 0.598418 |
Target: 5'- uCGGcuACGCCGcCGCC-CGGC-CCcAGCa -3' miRNA: 3'- -GUC--UGUGGCuGCGGaGCCGuGGcUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 60671 | 0.66 | 0.598418 |
Target: 5'- -uGACGC--ACGCgUUCuGCACCGAGCg -3' miRNA: 3'- guCUGUGgcUGCG-GAGcCGUGGCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 21166 | 0.66 | 0.598418 |
Target: 5'- -cGuCACCGACGgCagucgggucaUCGGCG-CGAGCa -3' miRNA: 3'- guCuGUGGCUGCgG----------AGCCGUgGCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 24226 | 0.66 | 0.587974 |
Target: 5'- -cGugACCGGCgggGCCgaGGCACgCGAGUu -3' miRNA: 3'- guCugUGGCUG---CGGagCCGUG-GCUCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 64768 | 0.66 | 0.587974 |
Target: 5'- -cGACGgUG-CGCCUCGGCGgcCCGcuGCg -3' miRNA: 3'- guCUGUgGCuGCGGAGCCGU--GGCu-CG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 34004 | 0.66 | 0.587974 |
Target: 5'- gAGcuGCaACCGGCuguauccgGCCUCGGCGgCGuGCg -3' miRNA: 3'- gUC--UG-UGGCUG--------CGGAGCCGUgGCuCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 37353 | 0.66 | 0.581723 |
Target: 5'- gGGuuGCCGcugugcgaguagcucACGcCCUCGGCACCGcccGCc -3' miRNA: 3'- gUCugUGGC---------------UGC-GGAGCCGUGGCu--CG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 38442 | 0.66 | 0.577563 |
Target: 5'- -cGAgACCGAaaaUGCCgugcgagGGCACCGAGg -3' miRNA: 3'- guCUgUGGCU---GCGGag-----CCGUGGCUCg -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 52131 | 0.66 | 0.577563 |
Target: 5'- --cGCACCGcCGUC-CGGCACCucGCc -3' miRNA: 3'- gucUGUGGCuGCGGaGCCGUGGcuCG- -5' |
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23264 | 3' | -59 | NC_005259.1 | + | 40072 | 0.66 | 0.577563 |
Target: 5'- -uGACccauggGCCGACGC----GCACCGAGCg -3' miRNA: 3'- guCUG------UGGCUGCGgagcCGUGGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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