Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23301 | 5' | -61.3 | NC_005259.1 | + | 26651 | 0.67 | 0.40567 |
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Target: 5'- --uGCGcucGGCAGGuuCGgCGCACCCg -3' miRNA: 3'- guuCGCca-CCGUCCcuGCgGCGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 20454 | 0.67 | 0.413597 |
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Target: 5'- aCGAGCcggGGUGGCucGGAugacCGCCGUcgaccucgacaccGCCCg -3' miRNA: 3'- -GUUCG---CCACCGucCCU----GCGGCG-------------UGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 41794 | 0.67 | 0.43155 |
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Target: 5'- gCGAGCaGUcGGCAGGcgguuugcacuccGACGagUGCACCCa -3' miRNA: 3'- -GUUCGcCA-CCGUCC-------------CUGCg-GCGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 11403 | 0.66 | 0.450882 |
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Target: 5'- uCAAGCGGUuucggcucguGGCcggugcaacGGGGAUGCuugggccgcuCGCugCCg -3' miRNA: 3'- -GUUCGCCA----------CCG---------UCCCUGCG----------GCGugGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 50278 | 0.66 | 0.450882 |
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Target: 5'- --cGuCGGUGGCGGGaACGUCGUGCa- -3' miRNA: 3'- guuC-GCCACCGUCCcUGCGGCGUGgg -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 55343 | 0.66 | 0.450882 |
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Target: 5'- -cAGCGGUGGC---GAUGCCGaaCGCCUg -3' miRNA: 3'- guUCGCCACCGuccCUGCGGC--GUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 25066 | 0.66 | 0.469727 |
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Target: 5'- uCAGGCaagGGUGGCGcugccaccGcCGCUGCGCCCg -3' miRNA: 3'- -GUUCG---CCACCGUcc------CuGCGGCGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 27999 | 0.66 | 0.479298 |
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Target: 5'- --uGCGGgugccggGGCGGGGuCGCCgggguuGCGCgCg -3' miRNA: 3'- guuCGCCa------CCGUCCCuGCGG------CGUGgG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 47952 | 0.66 | 0.479298 |
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Target: 5'- -cGGCGGUGcCGauGGcCGCCGCGCCg -3' miRNA: 3'- guUCGCCACcGUc-CCuGCGGCGUGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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