Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23301 | 5' | -61.3 | NC_005259.1 | + | 50438 | 0.69 | 0.3024 |
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Target: 5'- -cAGCGGUgcgGGCucGGGGuCGCCGguCUCg -3' miRNA: 3'- guUCGCCA---CCG--UCCCuGCGGCguGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 51642 | 0.72 | 0.189654 |
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Target: 5'- gGAGCGGUaGGCGaacGGGuuGCCGCGCg- -3' miRNA: 3'- gUUCGCCA-CCGU---CCCugCGGCGUGgg -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 55343 | 0.66 | 0.450882 |
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Target: 5'- -cAGCGGUGGC---GAUGCCGaaCGCCUg -3' miRNA: 3'- guUCGCCACCGuccCUGCGGC--GUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 58344 | 0.67 | 0.388401 |
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Target: 5'- uCAGGuCGGUGGCcucGGGcACGUCGCAg-- -3' miRNA: 3'- -GUUC-GCCACCGu--CCC-UGCGGCGUggg -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 58563 | 0.68 | 0.339573 |
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Target: 5'- uCGGGCGGUugGGCcGGaucgcGGCGCgGCugCCg -3' miRNA: 3'- -GUUCGCCA--CCGuCC-----CUGCGgCGugGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 58796 | 0.69 | 0.3024 |
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Target: 5'- gAGGCGGUgauugccucGGCGGGcucGCGCUgcucgGCACCCu -3' miRNA: 3'- gUUCGCCA---------CCGUCCc--UGCGG-----CGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 60425 | 0.71 | 0.226156 |
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Target: 5'- -cGGgGGUGGCccacGGGGCgGCCaCGCCCg -3' miRNA: 3'- guUCgCCACCGu---CCCUG-CGGcGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 61945 | 0.67 | 0.400439 |
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Target: 5'- --cGCgGGUGcCGGGGccaccgcagccucacGCGCCGCugCCu -3' miRNA: 3'- guuCG-CCACcGUCCC---------------UGCGGCGugGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 63645 | 0.69 | 0.309576 |
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Target: 5'- gCGAGCuGcucgcUGGCcGGGACGCCGgACaCCu -3' miRNA: 3'- -GUUCGcC-----ACCGuCCCUGCGGCgUG-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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