miRNA display CGI


Results 21 - 29 of 29 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23301 5' -61.3 NC_005259.1 + 25066 0.66 0.469727
Target:  5'- uCAGGCaagGGUGGCGcugccaccGcCGCUGCGCCCg -3'
miRNA:   3'- -GUUCG---CCACCGUcc------CuGCGGCGUGGG- -5'
23301 5' -61.3 NC_005259.1 + 24113 0.68 0.347393
Target:  5'- ---aUGGgaccgGGCGGGGAcuacacCGCCGcCGCCCu -3'
miRNA:   3'- guucGCCa----CCGUCCCU------GCGGC-GUGGG- -5'
23301 5' -61.3 NC_005259.1 + 21390 0.7 0.249583
Target:  5'- gAGGCGGUGcaacgcGCAuGGGACGCCcGUagACCUg -3'
miRNA:   3'- gUUCGCCAC------CGU-CCCUGCGG-CG--UGGG- -5'
23301 5' -61.3 NC_005259.1 + 20454 0.67 0.413597
Target:  5'- aCGAGCcggGGUGGCucGGAugacCGCCGUcgaccucgacaccGCCCg -3'
miRNA:   3'- -GUUCG---CCACCGucCCU----GCGGCG-------------UGGG- -5'
23301 5' -61.3 NC_005259.1 + 19851 0.68 0.379111
Target:  5'- --cGCGcucgguGUGGCGcucgcgcccgccGGGAucgccgaCGCCGCGCCCa -3'
miRNA:   3'- guuCGC------CACCGU------------CCCU-------GCGGCGUGGG- -5'
23301 5' -61.3 NC_005259.1 + 18789 1.1 0.000295
Target:  5'- gCAAGCGGUGGCAGGGACGCCGCACCCu -3'
miRNA:   3'- -GUUCGCCACCGUCCCUGCGGCGUGGG- -5'
23301 5' -61.3 NC_005259.1 + 18381 0.69 0.295352
Target:  5'- aGGGCGGUaGCgAGGGAgacgagcaccuCGCCGaGCCCg -3'
miRNA:   3'- gUUCGCCAcCG-UCCCU-----------GCGGCgUGGG- -5'
23301 5' -61.3 NC_005259.1 + 11403 0.66 0.450882
Target:  5'- uCAAGCGGUuucggcucguGGCcggugcaacGGGGAUGCuugggccgcuCGCugCCg -3'
miRNA:   3'- -GUUCGCCA----------CCG---------UCCCUGCG----------GCGugGG- -5'
23301 5' -61.3 NC_005259.1 + 6199 0.72 0.199521
Target:  5'- uGAGCaGcGUGaGCGGGGugagcCGCCGCGCCg -3'
miRNA:   3'- gUUCG-C-CAC-CGUCCCu----GCGGCGUGGg -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.