Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23330 | 5' | -54.8 | NC_005259.1 | + | 57691 | 0.74 | 0.343909 |
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Target: 5'- cCGuggCcAGGUUGCCGggcuugcccgacucGCGGGUCUGCCa -3' miRNA: 3'- -GCua-GuUCUAACGGU--------------CGCCCAGGCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 58283 | 0.77 | 0.251729 |
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Target: 5'- aGAUCAGGuucgcgagGCCAGCGGauaCCGCCa -3' miRNA: 3'- gCUAGUUCuaa-----CGGUCGCCca-GGCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 58790 | 0.73 | 0.416146 |
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Target: 5'- cCGGUCGAGGcggugaUUGCCucGGCGGGcUCgCGCUg -3' miRNA: 3'- -GCUAGUUCU------AACGG--UCGCCC-AG-GCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 60574 | 0.67 | 0.747482 |
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Target: 5'- aCGGUCAGGcg-GCCcacggcgAGuCGGGUgCCGUCa -3' miRNA: 3'- -GCUAGUUCuaaCGG-------UC-GCCCA-GGCGG- -5' |
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| 23330 | 5' | -54.8 | NC_005259.1 | + | 66491 | 0.75 | 0.299773 |
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Target: 5'- uCGAUCAugGGAUuuccuuccUGUCGGCGGGgcggucCCGCCu -3' miRNA: 3'- -GCUAGU--UCUA--------ACGGUCGCCCa-----GGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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