Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23354 | 5' | -54.5 | NC_005259.1 | + | 22856 | 0.69 | 0.591576 |
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Target: 5'- -cGCAGcCGCAACCUGuACUCGCA-GAUg -3' miRNA: 3'- gaUGUC-GUGUUGGGC-UGGGCGUuCUA- -5' |
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| 23354 | 5' | -54.5 | NC_005259.1 | + | 66242 | 0.7 | 0.546988 |
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Target: 5'- gUGCAGCcaucgacGCAGCgCGGCCCGCGccGGGc -3' miRNA: 3'- gAUGUCG-------UGUUGgGCUGGGCGU--UCUa -5' |
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| 23354 | 5' | -54.5 | NC_005259.1 | + | 9080 | 0.72 | 0.454649 |
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Target: 5'- -cACAGCAUAgGCCCGuuGCCCGcCGAGGg -3' miRNA: 3'- gaUGUCGUGU-UGGGC--UGGGC-GUUCUa -5' |
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| 23354 | 5' | -54.5 | NC_005259.1 | + | 67530 | 0.72 | 0.44479 |
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Target: 5'- -gGCGGCAaucACCUuGCCCGCGAGAUc -3' miRNA: 3'- gaUGUCGUgu-UGGGcUGGGCGUUCUA- -5' |
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| 23354 | 5' | -54.5 | NC_005259.1 | + | 56265 | 0.72 | 0.415955 |
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Target: 5'- cCUugAGCGCcgccGCCCGGCCCGUAu--- -3' miRNA: 3'- -GAugUCGUGu---UGGGCUGGGCGUucua -5' |
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| 23354 | 5' | -54.5 | NC_005259.1 | + | 27407 | 0.75 | 0.290318 |
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Target: 5'- -aGCGGCgGCAGCUCGguGCCCGCGAGGa -3' miRNA: 3'- gaUGUCG-UGUUGGGC--UGGGCGUUCUa -5' |
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| 23354 | 5' | -54.5 | NC_005259.1 | + | 42470 | 0.78 | 0.181164 |
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Target: 5'- aCUACGGCGaGGCCCGGCCCGU-AGAg -3' miRNA: 3'- -GAUGUCGUgUUGGGCUGGGCGuUCUa -5' |
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| 23354 | 5' | -54.5 | NC_005259.1 | + | 7371 | 0.8 | 0.145496 |
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Target: 5'- -gGCAGCACAACaCCucgggacaGACCCGCGAGAc -3' miRNA: 3'- gaUGUCGUGUUG-GG--------CUGGGCGUUCUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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