Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23388 | 3' | -64 | NC_005259.1 | + | 60137 | 0.68 | 0.232127 |
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Target: 5'- cGUCCuGCgCGgccuuGGCCGCCGCCUc -3' miRNA: 3'- -UAGGuCGgGCaguucCCGGCGGCGGA- -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 37234 | 0.68 | 0.220918 |
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Target: 5'- -gCUGGCCCGUgCcGGauuGGCUGCCGCCg -3' miRNA: 3'- uaGGUCGGGCA-GuUC---CCGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 68709 | 0.69 | 0.189553 |
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Target: 5'- --gCAGCgCGUCGgugcccgagaacuGGGGUCGCCGCUUg -3' miRNA: 3'- uagGUCGgGCAGU-------------UCCCGGCGGCGGA- -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 5422 | 0.69 | 0.210174 |
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Target: 5'- -gCC-GCCaCGUCGAGGucugcgcccGCUGCCGCCg -3' miRNA: 3'- uaGGuCGG-GCAGUUCC---------CGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 36633 | 0.69 | 0.213882 |
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Target: 5'- -gCC-GCCCG-CAAGGcccgcgaucagcgaGCCGCCGCCc -3' miRNA: 3'- uaGGuCGGGCaGUUCC--------------CGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 14856 | 0.7 | 0.165973 |
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Target: 5'- gGUCguGCUCG-CGAGGuucgcgcugcccgaGCCGCCGCCg -3' miRNA: 3'- -UAGguCGGGCaGUUCC--------------CGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 46858 | 0.7 | 0.158854 |
|
Target: 5'- aGUCCGcuGUCgGUCGAGGcuugaaucgcGCCGCCGCCc -3' miRNA: 3'- -UAGGU--CGGgCAGUUCC----------CGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 43935 | 0.7 | 0.158854 |
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Target: 5'- -aCCGcGCUacugacagCGUCGgcgaugggcGGGGCCGCCGCCUg -3' miRNA: 3'- uaGGU-CGG--------GCAGU---------UCCCGGCGGCGGA- -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 35608 | 0.74 | 0.081676 |
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Target: 5'- cGUCCuGGCCacgcugCGUCGAguaguucGGGCCGCCGCCg -3' miRNA: 3'- -UAGG-UCGG------GCAGUU-------CCCGGCGGCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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