Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23388 | 3' | -64 | NC_005259.1 | + | 29364 | 0.67 | 0.288575 |
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Target: 5'- cGUCgAGgcaCCCGUCGAGGucuacCCGCCGaCCUa -3' miRNA: 3'- -UAGgUC---GGGCAGUUCCc----GGCGGC-GGA- -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 45307 | 0.67 | 0.295473 |
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Target: 5'- -gCCGGUgaCGcCGAG-GCCGCCGCCg -3' miRNA: 3'- uaGGUCGg-GCaGUUCcCGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 45341 | 0.66 | 0.302499 |
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Target: 5'- -gCCuGCgCCG-CcuGGGCCGCCGCg- -3' miRNA: 3'- uaGGuCG-GGCaGuuCCCGGCGGCGga -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 26077 | 0.66 | 0.309652 |
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Target: 5'- --gCAGUCCccCGAG-GCCGCCGCCg -3' miRNA: 3'- uagGUCGGGcaGUUCcCGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 9674 | 0.66 | 0.316934 |
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Target: 5'- uUCCAcaugcgcuGCCgGUCGA-GGUCGCCGCUc -3' miRNA: 3'- uAGGU--------CGGgCAGUUcCCGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 19651 | 0.66 | 0.324344 |
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Target: 5'- -aCCGGCaCCGcCucGGGCaGCCGCUa -3' miRNA: 3'- uaGGUCG-GGCaGuuCCCGgCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 14663 | 0.66 | 0.331882 |
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Target: 5'- -cUCAGCCCGaCucGGGCCGCgUGCaCUc -3' miRNA: 3'- uaGGUCGGGCaGuuCCCGGCG-GCG-GA- -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 8746 | 0.66 | 0.339548 |
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Target: 5'- -gUCAGCCCGUCGAGG-UCGagGCCc -3' miRNA: 3'- uaGGUCGGGCAGUUCCcGGCggCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 39915 | 0.66 | 0.339548 |
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Target: 5'- -cUCGaUCCGUCGAGGGCCG-CGUCg -3' miRNA: 3'- uaGGUcGGGCAGUUCCCGGCgGCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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