Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23388 | 3' | -64 | NC_005259.1 | + | 43935 | 0.7 | 0.158854 |
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Target: 5'- -aCCGcGCUacugacagCGUCGgcgaugggcGGGGCCGCCGCCUg -3' miRNA: 3'- uaGGU-CGG--------GCAGU---------UCCCGGCGGCGGA- -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 45307 | 0.67 | 0.295473 |
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Target: 5'- -gCCGGUgaCGcCGAG-GCCGCCGCCg -3' miRNA: 3'- uaGGUCGg-GCaGUUCcCGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 45341 | 0.66 | 0.302499 |
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Target: 5'- -gCCuGCgCCG-CcuGGGCCGCCGCg- -3' miRNA: 3'- uaGGuCG-GGCaGuuCCCGGCGGCGga -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 45375 | 0.68 | 0.237909 |
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Target: 5'- gGUUuuGCCCGagGaauuucucGGGGUCGCCGCCg -3' miRNA: 3'- -UAGguCGGGCagU--------UCCCGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 46858 | 0.7 | 0.158854 |
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Target: 5'- aGUCCGcuGUCgGUCGAGGcuugaaucgcGCCGCCGCCc -3' miRNA: 3'- -UAGGU--CGGgCAGUUCC----------CGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 48484 | 0.67 | 0.261616 |
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Target: 5'- -gUCGGCCUGUUccucgacgcgcuuGAGGGCCGCgacuUGCCg -3' miRNA: 3'- uaGGUCGGGCAG-------------UUCCCGGCG----GCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 58697 | 0.67 | 0.27516 |
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Target: 5'- -gUCGGCUCGUC----GCCGCCGCCg -3' miRNA: 3'- uaGGUCGGGCAGuuccCGGCGGCGGa -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 60137 | 0.68 | 0.232127 |
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Target: 5'- cGUCCuGCgCGgccuuGGCCGCCGCCUc -3' miRNA: 3'- -UAGGuCGgGCaguucCCGGCGGCGGA- -5' |
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| 23388 | 3' | -64 | NC_005259.1 | + | 68709 | 0.69 | 0.189553 |
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Target: 5'- --gCAGCgCGUCGgugcccgagaacuGGGGUCGCCGCUUg -3' miRNA: 3'- uagGUCGgGCAGU-------------UCCCGGCGGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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