Results 1 - 20 of 90 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23436 | 3' | -65.8 | NC_005259.1 | + | 49633 | 0.74 | 0.085888 |
Target: 5'- uCGGCGGcaGCCGC-GUCGGcgAUGGCGGCc -3' miRNA: 3'- -GCCGCC--CGGUGgCGGCCa-UGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 17340 | 0.66 | 0.33044 |
Target: 5'- cCGGUcaacGUCGCCGUCgaGGU-CGGCGGCu -3' miRNA: 3'- -GCCGcc--CGGUGGCGG--CCAuGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 4482 | 0.71 | 0.134274 |
Target: 5'- uCGGCGGuggaccauggugaucGCCACCgcGCCGGgagugGCGcugcGCGGCa -3' miRNA: 3'- -GCCGCC---------------CGGUGG--CGGCCa----UGC----CGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 13086 | 0.71 | 0.132922 |
Target: 5'- cCGGCcauccGGuGCCACCGaccCCGGcccgcuCGGCGGCu -3' miRNA: 3'- -GCCG-----CC-CGGUGGC---GGCCau----GCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 62719 | 0.72 | 0.129595 |
Target: 5'- aGGCGGGggaucUCGCuCGaCCGGacCGGCGGCa -3' miRNA: 3'- gCCGCCC-----GGUG-GC-GGCCauGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 60145 | 0.72 | 0.126346 |
Target: 5'- gCGGCcuuGGCCGCCGCCucagcGGccucACgGGCGGCg -3' miRNA: 3'- -GCCGc--CCGGUGGCGG-----CCa---UG-CCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 25906 | 0.72 | 0.123172 |
Target: 5'- aGGauGGCCgccugagccgccGCCGCCGGUcCGGUGaGCg -3' miRNA: 3'- gCCgcCCGG------------UGGCGGCCAuGCCGC-CG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 29947 | 0.72 | 0.111205 |
Target: 5'- gCGGCccGGUCGCgGCUGGUGgGGCGGg -3' miRNA: 3'- -GCCGc-CCGGUGgCGGCCAUgCCGCCg -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 60286 | 0.73 | 0.108109 |
Target: 5'- gGGC-GGCCAucgUCGUCGGUGCGGCcacggacGGCg -3' miRNA: 3'- gCCGcCCGGU---GGCGGCCAUGCCG-------CCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 13419 | 0.73 | 0.105637 |
Target: 5'- uGGCcgGGGUCGCCGCCGGggccGCGcuaGGCg -3' miRNA: 3'- gCCG--CCCGGUGGCGGCCa---UGCcg-CCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 40134 | 0.73 | 0.097775 |
Target: 5'- uGGCGGGCgagcuCGCCGUCGGUuguCGGUgcccgccccGGCa -3' miRNA: 3'- gCCGCCCG-----GUGGCGGCCAu--GCCG---------CCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 67981 | 0.84 | 0.014674 |
Target: 5'- gCGGCGuGGCCACCGCCGcgagcGUcgacuugcccuccuGCGGCGGCa -3' miRNA: 3'- -GCCGC-CCGGUGGCGGC-----CA--------------UGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 37045 | 0.74 | 0.083682 |
Target: 5'- uGGUGcccGCCACCGCCc--ACGGCGGCg -3' miRNA: 3'- gCCGCc--CGGUGGCGGccaUGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 8334 | 0.74 | 0.082815 |
Target: 5'- aCGGCGaucagccggucacGCUGCUGCCGGUAucgcuCGGCGGCg -3' miRNA: 3'- -GCCGCc------------CGGUGGCGGCCAU-----GCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 66619 | 0.74 | 0.082384 |
Target: 5'- gGGCGguauuucucgucccuGGCCACCgcucggauugcuGCCGGU-CGGCGGUg -3' miRNA: 3'- gCCGC---------------CCGGUGG------------CGGCCAuGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 29999 | 0.74 | 0.082384 |
Target: 5'- aCGGUGGucaccacgacaagguGaCCACCGCCGGgGCGGUGGg -3' miRNA: 3'- -GCCGCC---------------C-GGUGGCGGCCaUGCCGCCg -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 66258 | 0.75 | 0.071539 |
Target: 5'- -aGCGcGGCC-CgCGCCGG-GCGGCGGCu -3' miRNA: 3'- gcCGC-CCGGuG-GCGGCCaUGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 5711 | 0.75 | 0.067881 |
Target: 5'- -cGCcaaGGCCAgCGCCGG-ACGGCGGCc -3' miRNA: 3'- gcCGc--CCGGUgGCGGCCaUGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 31249 | 0.76 | 0.057958 |
Target: 5'- gCGGCGGuGCCGCCccacacGCCGcaguagGCGGUGGCg -3' miRNA: 3'- -GCCGCC-CGGUGG------CGGCca----UGCCGCCG- -5' |
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23436 | 3' | -65.8 | NC_005259.1 | + | 738 | 0.76 | 0.057349 |
Target: 5'- cCGGCGGuGCCuacGCCGCCGGUgccuaccGCguccaccgcgccgaGGCGGCu -3' miRNA: 3'- -GCCGCC-CGG---UGGCGGCCA-------UG--------------CCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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