Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23460 | 3' | -60.3 | NC_005259.1 | + | 24907 | 0.67 | 0.450882 |
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Target: 5'- cGGCGaGCgGGuCUACaCCGAauaUCAGGa -3' miRNA: 3'- -CCGCgCGgCCuGAUG-GGCUg--AGUCCa -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 15793 | 0.67 | 0.479298 |
|
Target: 5'- uGGC-CGCCGGGaucgaccGCCCGACaUCcaAGGUg -3' miRNA: 3'- -CCGcGCGGCCUga-----UGGGCUG-AG--UCCA- -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 14654 | 0.71 | 0.26203 |
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Target: 5'- uGGCGU---GGGCUcaGCCCGACUCGGGc -3' miRNA: 3'- -CCGCGcggCCUGA--UGGGCUGAGUCCa -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 11005 | 0.71 | 0.288433 |
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Target: 5'- cGGCaccGCGCCGGAC-GCgCCGAggCAGGa -3' miRNA: 3'- -CCG---CGCGGCCUGaUG-GGCUgaGUCCa -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 8952 | 0.68 | 0.414484 |
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Target: 5'- gGGCGCGCCguGGACggGCaCCGuGC-CGGGa -3' miRNA: 3'- -CCGCGCGG--CCUGa-UG-GGC-UGaGUCCa -5' |
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| 23460 | 3' | -60.3 | NC_005259.1 | + | 7448 | 0.66 | 0.558887 |
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Target: 5'- uGGCaGCGCCGaggauggcuuGACUugacaauCCCGACauaggCGGGUa -3' miRNA: 3'- -CCG-CGCGGC----------CUGAu------GGGCUGa----GUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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