Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23510 | 3' | -58.5 | NC_005259.1 | + | 42459 | 0.66 | 0.642914 |
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Target: 5'- aACaGUcGCCGACuaCGGCGAGGccCGGc -3' miRNA: 3'- cUGcCA-CGGCUGuaGCCGCUCCa-GCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 61049 | 0.66 | 0.652311 |
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Target: 5'- cGACGGgcggcucaccuugUGCCgGGCAUCggcauaGGCGucGUCGGc -3' miRNA: 3'- -CUGCC-------------ACGG-CUGUAG------CCGCucCAGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 14540 | 0.66 | 0.662736 |
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Target: 5'- -uCGGUGCCGAguUCGuccggcaggccgaGCGGGGacCGGc -3' miRNA: 3'- cuGCCACGGCUguAGC-------------CGCUCCa-GCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 33342 | 0.66 | 0.663777 |
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Target: 5'- uGAuCGGgGUCGAUgcaAUCgGGCGAGGUCa- -3' miRNA: 3'- -CU-GCCaCGGCUG---UAG-CCGCUCCAGcc -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 36368 | 0.66 | 0.663777 |
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Target: 5'- --aGGgauugcGUCGAag-CGGCGAGGUUGGc -3' miRNA: 3'- cugCCa-----CGGCUguaGCCGCUCCAGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 41195 | 0.66 | 0.663777 |
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Target: 5'- cGAUGGUgauGCCGuuGUCGGUGuGGaUCGu -3' miRNA: 3'- -CUGCCA---CGGCugUAGCCGCuCC-AGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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