Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23510 | 3' | -58.5 | NC_005259.1 | + | 54795 | 0.74 | 0.24933 |
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Target: 5'- uGCGGUGCCGACcguUCGGUaGGGGaUCGu -3' miRNA: 3'- cUGCCACGGCUGu--AGCCG-CUCC-AGCc -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 64359 | 0.74 | 0.237345 |
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Target: 5'- gGGCGGUGCC--CAUCaGCGAGGUCu- -3' miRNA: 3'- -CUGCCACGGcuGUAGcCGCUCCAGcc -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 60754 | 0.74 | 0.237345 |
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Target: 5'- uGCaGUGCaguCGGC-UCGGCGAGGUUGGg -3' miRNA: 3'- cUGcCACG---GCUGuAGCCGCUCCAGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 27394 | 0.75 | 0.209486 |
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Target: 5'- cGGCGGUGUCGGCAgCGGCGgcAGcUCGGu -3' miRNA: 3'- -CUGCCACGGCUGUaGCCGC--UCcAGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 8371 | 0.75 | 0.209486 |
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Target: 5'- cGGCGGcgGCCucGGCAUCGGCGAGaGccUCGGc -3' miRNA: 3'- -CUGCCa-CGG--CUGUAGCCGCUC-C--AGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 60565 | 0.75 | 0.197144 |
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Target: 5'- -cCGGUGCCGACGgucaggcggcccaCGGCGA-GUCGGg -3' miRNA: 3'- cuGCCACGGCUGUa------------GCCGCUcCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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