Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23514 | 5' | -57.8 | NC_005259.1 | + | 36442 | 0.72 | 0.342814 |
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Target: 5'- gUCGCCGA-GGUCga--CCGCGUCGAg -3' miRNA: 3'- gAGCGGCUgCCAGaaccGGUGCAGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 60568 | 0.72 | 0.334972 |
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Target: 5'- -gUGCCGACGGUCaggcGGCCcACGgCGAg -3' miRNA: 3'- gaGCGGCUGCCAGaa--CCGG-UGCaGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 46611 | 0.72 | 0.334195 |
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Target: 5'- gUCGuuGGCGGUCUUGGUggccugaCugGUCa- -3' miRNA: 3'- gAGCggCUGCCAGAACCG-------GugCAGcu -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 58913 | 0.73 | 0.304926 |
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Target: 5'- gCUCGUCGGCGGUCUUGu---CGUCGGc -3' miRNA: 3'- -GAGCGGCUGCCAGAACcgguGCAGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 39900 | 0.74 | 0.238379 |
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Target: 5'- gUCGCCGcCGcccgccucgauccGUCgagGGCCGCGUCGAg -3' miRNA: 3'- gAGCGGCuGC-------------CAGaa-CCGGUGCAGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 42941 | 0.77 | 0.162769 |
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Target: 5'- cCUCGCCGugGG-CgaGGCCGCcguuGUCGAa -3' miRNA: 3'- -GAGCGGCugCCaGaaCCGGUG----CAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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