Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23579 | 3' | -56.1 | NC_005261.1 | + | 32273 | 0.66 | 0.891131 |
Target: 5'- cCGAggcgccGCCGCUGGUgcUGGAGGCgGGc--- -3' miRNA: 3'- -GCU------UGGCGACCG--ACCUCUGgCUcaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 111077 | 0.66 | 0.891131 |
Target: 5'- aGGAUCGagggcCUGGUgugGGGGGCCGGGg-- -3' miRNA: 3'- gCUUGGC-----GACCGa--CCUCUGGCUCaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 136790 | 0.66 | 0.891131 |
Target: 5'- cCGAggcgccGCCGCUGGUgcUGGAGGCgGGc--- -3' miRNA: 3'- -GCU------UGGCGACCG--ACCUCUGgCUcaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 67542 | 0.66 | 0.891131 |
Target: 5'- gCGGGCCGCgcuggacgccUGGCUGGAgGugCGGc--- -3' miRNA: 3'- -GCUUGGCG----------ACCGACCU-CugGCUcaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 25602 | 0.66 | 0.891131 |
Target: 5'- cCGGGCCGUgcagccGGCUGaGGugCGAGgcgcggUCg -3' miRNA: 3'- -GCUUGGCGa-----CCGACcUCugGCUCa-----AG- -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 14509 | 0.66 | 0.891131 |
Target: 5'- gCGGACCGCgacgacGCUGcuGAuGGCCGAGUa- -3' miRNA: 3'- -GCUUGGCGac----CGAC--CU-CUGGCUCAag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 98065 | 0.66 | 0.889096 |
Target: 5'- gGGACCGCgGGCggcggguguguccuUGGAGAauCUGGGggCg -3' miRNA: 3'- gCUUGGCGaCCG--------------ACCUCU--GGCUCaaG- -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 54644 | 0.66 | 0.889096 |
Target: 5'- gCGAcgcGCUGCUGGCggccgcgGGcGccgcgggcacgcccGCCGAGUUCu -3' miRNA: 3'- -GCU---UGGCGACCGa------CCuC--------------UGGCUCAAG- -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 119758 | 0.66 | 0.884266 |
Target: 5'- uGAACCGCUGGCau---ACCGccAGUUCu -3' miRNA: 3'- gCUUGGCGACCGaccucUGGC--UCAAG- -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 95572 | 0.66 | 0.880039 |
Target: 5'- cCGGccGCCGCuUGGCUGGuuccgcgcggccgcgGcGGCCGAGUc- -3' miRNA: 3'- -GCU--UGGCG-ACCGACC---------------U-CUGGCUCAag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 96826 | 0.66 | 0.877176 |
Target: 5'- aGAGCUGUcGGCggugucGAGGCCGGGgUCg -3' miRNA: 3'- gCUUGGCGaCCGac----CUCUGGCUCaAG- -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 34684 | 0.66 | 0.877176 |
Target: 5'- gGGGCCcCUgGGCccGGGGGCCGAGg-- -3' miRNA: 3'- gCUUGGcGA-CCGa-CCUCUGGCUCaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 128634 | 0.66 | 0.869864 |
Target: 5'- cCGGGCCcgagGCggGGgUGGGGGCUGGGUg- -3' miRNA: 3'- -GCUUGG----CGa-CCgACCUCUGGCUCAag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 72244 | 0.66 | 0.869864 |
Target: 5'- gCGGACCuGgUGGCcguUGGGGACCGgcuAGUg- -3' miRNA: 3'- -GCUUGG-CgACCG---ACCUCUGGC---UCAag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 18699 | 0.66 | 0.869864 |
Target: 5'- gGGGCCuuGCUcGGCcgcGGGGACCGGGg-- -3' miRNA: 3'- gCUUGG--CGA-CCGa--CCUCUGGCUCaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 78557 | 0.66 | 0.869864 |
Target: 5'- gCGAuCCGCUcGCgcgccgGGAccGCCGAGUUCg -3' miRNA: 3'- -GCUuGGCGAcCGa-----CCUc-UGGCUCAAG- -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 24116 | 0.66 | 0.869864 |
Target: 5'- cCGGGCCcgagGCggGGgUGGGGGCUGGGUg- -3' miRNA: 3'- -GCUUGG----CGa-CCgACCUCUGGCUCAag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 32986 | 0.67 | 0.862337 |
Target: 5'- gGGACCGCgGaGgaGGGGGCCGAc--- -3' miRNA: 3'- gCUUGGCGaC-CgaCCUCUGGCUcaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 22148 | 0.67 | 0.862337 |
Target: 5'- gCGGACCGC-GGCauccacGGGGACCGGc--- -3' miRNA: 3'- -GCUUGGCGaCCGa-----CCUCUGGCUcaag -5' |
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23579 | 3' | -56.1 | NC_005261.1 | + | 137504 | 0.67 | 0.862337 |
Target: 5'- gGGACCGCgGaGgaGGGGGCCGAc--- -3' miRNA: 3'- gCUUGGCGaC-CgaCCUCUGGCUcaag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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