Results 1 - 20 of 91 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23580 | 5' | -56.4 | NC_005261.1 | + | 82490 | 0.65 | 0.861355 |
Target: 5'- ---uCGAGGCCGuccggcagcacGCCCGcGACAUAg -3' miRNA: 3'- aaauGCUCCGGCuca--------CGGGC-CUGUAU- -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 53855 | 0.66 | 0.831999 |
Target: 5'- --gGCGGcGGCCGAGgccgaGCCCgcGGGCGg- -3' miRNA: 3'- aaaUGCU-CCGGCUCa----CGGG--CCUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 35305 | 0.66 | 0.848634 |
Target: 5'- --aGCGccGGGCCGGGcUGCUCGGGgAg- -3' miRNA: 3'- aaaUGC--UCCGGCUC-ACGGGCCUgUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 113910 | 0.66 | 0.840415 |
Target: 5'- --cGCGAuGGCCGAG-GCagCGGGCGc- -3' miRNA: 3'- aaaUGCU-CCGGCUCaCGg-GCCUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 30483 | 0.66 | 0.848634 |
Target: 5'- --gAgGAGGCCGGG-GCCuCGGAa--- -3' miRNA: 3'- aaaUgCUCCGGCUCaCGG-GCCUguau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 125719 | 0.66 | 0.823394 |
Target: 5'- --cGCGGGGCCccgcgcGGUcGCCCGGgggGCAUGg -3' miRNA: 3'- aaaUGCUCCGGc-----UCA-CGGGCC---UGUAU- -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 127998 | 0.66 | 0.856648 |
Target: 5'- --gGCGAGuGCgCGGGcccGCCUGGGCAg- -3' miRNA: 3'- aaaUGCUC-CG-GCUCa--CGGGCCUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 47244 | 0.66 | 0.856648 |
Target: 5'- --gACGAGGCCGAGguacuucuuCuuGGugAUGa -3' miRNA: 3'- aaaUGCUCCGGCUCac-------GggCCugUAU- -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 54089 | 0.66 | 0.840415 |
Target: 5'- --gGCGuGGCCGAG-GCCCGccaGCGa- -3' miRNA: 3'- aaaUGCuCCGGCUCaCGGGCc--UGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 129699 | 0.66 | 0.840415 |
Target: 5'- ---cCGGGGCCGGG-GCCgGaGGCAg- -3' miRNA: 3'- aaauGCUCCGGCUCaCGGgC-CUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 135000 | 0.66 | 0.848634 |
Target: 5'- --gAgGAGGCCGGG-GCCuCGGAa--- -3' miRNA: 3'- aaaUgCUCCGGCUCaCGG-GCCUguau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 59076 | 0.66 | 0.848634 |
Target: 5'- --cACGGGGCUGcuGGUGCucgCCGGGCu-- -3' miRNA: 3'- aaaUGCUCCGGC--UCACG---GGCCUGuau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 107495 | 0.66 | 0.840415 |
Target: 5'- --gGCuGAGGCUGGG-GCUCGGGCc-- -3' miRNA: 3'- aaaUG-CUCCGGCUCaCGGGCCUGuau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 11698 | 0.66 | 0.840415 |
Target: 5'- --cGCGuGGCCGAGgcgcggcggGCgCGGGCGc- -3' miRNA: 3'- aaaUGCuCCGGCUCa--------CGgGCCUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 94822 | 0.66 | 0.848634 |
Target: 5'- --gGCgGAGGCCGcccccGGcGCCUGGACGg- -3' miRNA: 3'- aaaUG-CUCCGGC-----UCaCGGGCCUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 39942 | 0.66 | 0.831999 |
Target: 5'- ---cCGAGGCCGcGgacGCgCCGGGCGUc -3' miRNA: 3'- aaauGCUCCGGCuCa--CG-GGCCUGUAu -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 23481 | 0.66 | 0.856648 |
Target: 5'- --gGCGAGuGCgCGGGcccGCCUGGGCAg- -3' miRNA: 3'- aaaUGCUC-CG-GCUCa--CGGGCCUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 90398 | 0.66 | 0.856648 |
Target: 5'- --gGCGGGGCUG-GcGCCCGGcGCGg- -3' miRNA: 3'- aaaUGCUCCGGCuCaCGGGCC-UGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 105758 | 0.66 | 0.856648 |
Target: 5'- --cGCGGGGaCCuGGGUGCUCaGGGCGc- -3' miRNA: 3'- aaaUGCUCC-GG-CUCACGGG-CCUGUau -5' |
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23580 | 5' | -56.4 | NC_005261.1 | + | 133816 | 0.66 | 0.840415 |
Target: 5'- --gGCGAGGCCug--GCCCGG-CAg- -3' miRNA: 3'- aaaUGCUCCGGcucaCGGGCCuGUau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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