Results 1 - 20 of 89 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23592 | 5' | -59.7 | NC_005261.1 | + | 1938 | 0.68 | 0.571341 |
Target: 5'- cCGCgGGcaGCaCCGcGgCGCGCAGGUACa -3' miRNA: 3'- -GCGaCUa-CG-GGCaCgGCGCGUCCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 2200 | 0.71 | 0.447068 |
Target: 5'- aGCcGGuUGCCCaGcGCCGCgaGCAGGUGCg -3' miRNA: 3'- gCGaCU-ACGGG-CaCGGCG--CGUCCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 3615 | 0.67 | 0.63176 |
Target: 5'- aGCUcaGcUGCCCGgaGCaCGCGCuccGGGUGCg -3' miRNA: 3'- gCGA--CuACGGGCa-CG-GCGCG---UCCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 12123 | 0.66 | 0.721824 |
Target: 5'- gCGCcagGAUggcGCCCGccgccgcggcgGCCGCGCAGGc-- -3' miRNA: 3'- -GCGa--CUA---CGGGCa----------CGGCGCGUCCaug -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 13643 | 0.66 | 0.692167 |
Target: 5'- aCGCgGAagcgcGCgCCGUGCCGCcgccGCGGGggggGCg -3' miRNA: 3'- -GCGaCUa----CG-GGCACGGCG----CGUCCa---UG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 13943 | 0.69 | 0.541606 |
Target: 5'- gCGUUGcgGCCCa-GCCGCGCGGaaACg -3' miRNA: 3'- -GCGACuaCGGGcaCGGCGCGUCcaUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 14767 | 0.7 | 0.502813 |
Target: 5'- cCGCgacagGggGgCCGccCCGCGCAGGUGCc -3' miRNA: 3'- -GCGa----CuaCgGGCacGGCGCGUCCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 19249 | 0.66 | 0.73157 |
Target: 5'- uGCgGggGCgCUGaacaGCgCGCGCGGGUACg -3' miRNA: 3'- gCGaCuaCG-GGCa---CG-GCGCGUCCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 19403 | 0.66 | 0.721824 |
Target: 5'- uCGgUGAUcgGCCCGUguGCCGCGCcgaagucgcccAGGcACa -3' miRNA: 3'- -GCgACUA--CGGGCA--CGGCGCG-----------UCCaUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 20571 | 0.68 | 0.591383 |
Target: 5'- gCGCaGcgGCgCCG-GCgGCGCGGGUGg -3' miRNA: 3'- -GCGaCuaCG-GGCaCGgCGCGUCCAUg -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 20727 | 0.66 | 0.720845 |
Target: 5'- gCGCUGggGCUCG-GCCGUuucacguGUGGGUGu -3' miRNA: 3'- -GCGACuaCGGGCaCGGCG-------CGUCCAUg -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 23202 | 0.69 | 0.551466 |
Target: 5'- cCGC-GggGCCCGcagcggcgGCCGCGCGcaaaagccGGUGCa -3' miRNA: 3'- -GCGaCuaCGGGCa-------CGGCGCGU--------CCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 29426 | 0.66 | 0.73157 |
Target: 5'- cCGC-GggGCCCGcgGCCGUGUucGUGCc -3' miRNA: 3'- -GCGaCuaCGGGCa-CGGCGCGucCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 29641 | 0.66 | 0.720845 |
Target: 5'- cCGCUGccGCCgGUcggggacgccaugGCCGCGCAGa--- -3' miRNA: 3'- -GCGACuaCGGgCA-------------CGGCGCGUCcaug -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 29843 | 0.67 | 0.641874 |
Target: 5'- aGCUGcgcgccGUGCUCGccgGCCGCGCGGcGccgGCg -3' miRNA: 3'- gCGAC------UACGGGCa--CGGCGCGUC-Ca--UG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 29944 | 0.66 | 0.721824 |
Target: 5'- gCGCUGcgGCC--UGCCGCG-AGGcGCu -3' miRNA: 3'- -GCGACuaCGGgcACGGCGCgUCCaUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 30774 | 0.66 | 0.692167 |
Target: 5'- gGCcGGaGCCCG-GCCGCgGCgguggAGGUGCu -3' miRNA: 3'- gCGaCUaCGGGCaCGGCG-CG-----UCCAUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 31283 | 0.73 | 0.310156 |
Target: 5'- gCGCUGGccgagaucgcgGCCCGccGCCGCGCGGGgcccGCg -3' miRNA: 3'- -GCGACUa----------CGGGCa-CGGCGCGUCCa---UG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 31738 | 0.68 | 0.611542 |
Target: 5'- gCGC-GAUgGCCCG-GCCGUcggGCAGGcGCa -3' miRNA: 3'- -GCGaCUA-CGGGCaCGGCG---CGUCCaUG- -5' |
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23592 | 5' | -59.7 | NC_005261.1 | + | 31994 | 0.78 | 0.170326 |
Target: 5'- cCGC-GGUGCCCGUGCCGcCGCGcGaGUACc -3' miRNA: 3'- -GCGaCUACGGGCACGGC-GCGU-C-CAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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