Results 1 - 20 of 60 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23823 | 5' | -62.6 | NC_005261.1 | + | 84952 | 0.65 | 0.626291 |
Target: 5'- cCUCG-GCGGccgccgaggcgucGGGCGcGUCCGCGAuGCGc -3' miRNA: 3'- -GAGCaCGCCc------------CCCGC-UAGGCGCU-CGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 60052 | 0.66 | 0.620397 |
Target: 5'- -aCGgcgGCGGcgaagagcGGcGGcCGGUCCGCGGGCu -3' miRNA: 3'- gaGCa--CGCC--------CC-CC-GCUAGGCGCUCGu -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 137752 | 0.66 | 0.620397 |
Target: 5'- aUCGgcgcGCGcGGGGGCGGggUGCgGGGCGc -3' miRNA: 3'- gAGCa---CGC-CCCCCGCUagGCG-CUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 69 | 0.66 | 0.620397 |
Target: 5'- aUCGgcgcGCGcGGGGGCGGggUGCgGGGCGc -3' miRNA: 3'- gAGCa---CGC-CCCCCGCUagGCG-CUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 125269 | 0.66 | 0.616468 |
Target: 5'- cCUCGUcCGGGGGcCGcuggaagucaggCCGCGGGCGc -3' miRNA: 3'- -GAGCAcGCCCCCcGCua----------GGCGCUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 23176 | 0.66 | 0.610579 |
Target: 5'- -gCGcGCcGGGGGCGAca-GCGAGCGc -3' miRNA: 3'- gaGCaCGcCCCCCGCUaggCGCUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 69227 | 0.66 | 0.610579 |
Target: 5'- -cUGUGCGcgcuGGGGCGGUUCGcCGAgGCGc -3' miRNA: 3'- gaGCACGCc---CCCCGCUAGGC-GCU-CGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 129657 | 0.66 | 0.610579 |
Target: 5'- -gCGgcgGCGGcaGGGGCGGgaCCGgGGGCGu -3' miRNA: 3'- gaGCa--CGCC--CCCCGCUa-GGCgCUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 34933 | 0.66 | 0.610579 |
Target: 5'- ----cGCGGGGGGCGG-CCGgcgcgaccCGGGCc -3' miRNA: 3'- gagcaCGCCCCCCGCUaGGC--------GCUCGu -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 67069 | 0.66 | 0.604696 |
Target: 5'- -gCGcGCGGGggcucgcucGGGCGGUCCauggcgcgacgcgguGCGGGCGg -3' miRNA: 3'- gaGCaCGCCC---------CCCGCUAGG---------------CGCUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 95812 | 0.66 | 0.600777 |
Target: 5'- -cCG-GCGGGGGcucGCGGUCCucgcggcgcuuGUGGGCGg -3' miRNA: 3'- gaGCaCGCCCCC---CGCUAGG-----------CGCUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 127811 | 0.66 | 0.600777 |
Target: 5'- -gCG-GCGGcGGGccgcucgcgcucGCGG-CCGCGAGCAg -3' miRNA: 3'- gaGCaCGCC-CCC------------CGCUaGGCGCUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 97652 | 0.66 | 0.59002 |
Target: 5'- --gGUGCcgcaccuGGGGGGCcggcGUCCGCGGGg- -3' miRNA: 3'- gagCACG-------CCCCCCGc---UAGGCGCUCgu -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 46104 | 0.66 | 0.581244 |
Target: 5'- -aCG-GcCGGGGGccGUGGUCCGCGuccAGCAg -3' miRNA: 3'- gaGCaC-GCCCCC--CGCUAGGCGC---UCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 81364 | 0.66 | 0.581244 |
Target: 5'- ---uUGCGGGGGGCGccgggcucgCUGgGGGCGc -3' miRNA: 3'- gagcACGCCCCCCGCua-------GGCgCUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 71962 | 0.66 | 0.581244 |
Target: 5'- gUCG-GCGcGGGGcGCGGagCGCGcGGCAg -3' miRNA: 3'- gAGCaCGC-CCCC-CGCUagGCGC-UCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 56113 | 0.66 | 0.571524 |
Target: 5'- -gCGaGCGGcGGGaGCGcgCCGCaGGGCGg -3' miRNA: 3'- gaGCaCGCC-CCC-CGCuaGGCG-CUCGU- -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 105271 | 0.66 | 0.571524 |
Target: 5'- gCUCG-GCGGGcGGCaGcgCCGgCGAGCc -3' miRNA: 3'- -GAGCaCGCCCcCCG-CuaGGC-GCUCGu -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 69763 | 0.67 | 0.561844 |
Target: 5'- ----cGCGGGGGGCGGcgCCGgggacUGAGCc -3' miRNA: 3'- gagcaCGCCCCCCGCUa-GGC-----GCUCGu -5' |
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23823 | 5' | -62.6 | NC_005261.1 | + | 78614 | 0.67 | 0.561844 |
Target: 5'- aCUCGgcUGUGGucGGGGCGccagGUCCGcCGAGUu -3' miRNA: 3'- -GAGC--ACGCC--CCCCGC----UAGGC-GCUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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