Results 1 - 20 of 105 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23834 | 3' | -50.7 | NC_005261.1 | + | 19933 | 0.66 | 0.995955 |
Target: 5'- gCCCucgcuaGCGUccUGGGCGggGGu----GGGGc -3' miRNA: 3'- -GGG------CGCA--ACCCGCuuUUuucuuCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 136182 | 0.66 | 0.995955 |
Target: 5'- --aGCGaggaaaaggGGGCGAG--GAGAAGGGa -3' miRNA: 3'- gggCGCaa-------CCCGCUUuuUUCUUCCCc -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 6411 | 0.66 | 0.995955 |
Target: 5'- gCgCGCGUUGGcccggccugcucGCGAGGGcGGuccGGGGGc -3' miRNA: 3'- -GgGCGCAACC------------CGCUUUUuUCu--UCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 28100 | 0.66 | 0.995955 |
Target: 5'- gCCGCagcaaagGGGUu--AGAGGggGGGGc -3' miRNA: 3'- gGGCGcaa----CCCGcuuUUUUCuuCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 57145 | 0.66 | 0.995955 |
Target: 5'- aUCGUGUuuauuggcaUGGGgGGAAAugGAGAGGaGGGn -3' miRNA: 3'- gGGCGCA---------ACCCgCUUUU--UUCUUC-CCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 73824 | 0.66 | 0.995891 |
Target: 5'- gCCCGCGccuagcccggcucUUGGGCGAGucggcgccccGGAGGcuuuuAAGcGGGu -3' miRNA: 3'- -GGGCGC-------------AACCCGCUU----------UUUUC-----UUC-CCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 69294 | 0.66 | 0.995826 |
Target: 5'- cCCCgGCGcgGGGCGccgguggcggcGGGAGcGGGGGu -3' miRNA: 3'- -GGG-CGCaaCCCGCuu---------UUUUCuUCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 119704 | 0.66 | 0.995276 |
Target: 5'- uCCaCGCGc--GGCGAu--GAGGAGGGc -3' miRNA: 3'- -GG-GCGCaacCCGCUuuuUUCUUCCCc -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 119273 | 0.66 | 0.995203 |
Target: 5'- uCCUGCucgcUGGGCGggGgcucgccGAGGAAGcGGa -3' miRNA: 3'- -GGGCGca--ACCCGCuuU-------UUUCUUC-CCc -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 134363 | 0.66 | 0.995203 |
Target: 5'- gCCgCGCGggGGGCGGcgccgcuggcgacGGcGAGAGcGGGa -3' miRNA: 3'- -GG-GCGCaaCCCGCU-------------UUuUUCUUcCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 13086 | 0.66 | 0.994506 |
Target: 5'- gCCCGuCGgcGGGCGGcgGGcucGGcucGGGGGc -3' miRNA: 3'- -GGGC-GCaaCCCGCUuuUU---UCu--UCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 56107 | 0.66 | 0.993638 |
Target: 5'- uCCUGCGcgaGcGGCGGGAGcgcgccgcAGGgcGGGGg -3' miRNA: 3'- -GGGCGCaa-C-CCGCUUUU--------UUCuuCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 15026 | 0.66 | 0.992664 |
Target: 5'- gUCgGCGggugccggGGGCGuagc-GGggGGGGu -3' miRNA: 3'- -GGgCGCaa------CCCGCuuuuuUCuuCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 120928 | 0.67 | 0.991573 |
Target: 5'- aUCCGCGagcgccGGGCGc---GGGAAGGuGGa -3' miRNA: 3'- -GGGCGCaa----CCCGCuuuuUUCUUCC-CC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 48018 | 0.67 | 0.991573 |
Target: 5'- cCCCGcCGgcagagcaGGGCGAGGAGcacgcgcgcgcGGucGGGGc -3' miRNA: 3'- -GGGC-GCaa------CCCGCUUUUU-----------UCuuCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 16178 | 0.67 | 0.991573 |
Target: 5'- -gCGCGggcGuGGCGggGGuGGgcGGGGg -3' miRNA: 3'- ggGCGCaa-C-CCGCuuUUuUCuuCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 74343 | 0.67 | 0.990359 |
Target: 5'- aCCGCaGgcGcGGCGGc---GGAAGGGGc -3' miRNA: 3'- gGGCG-CaaC-CCGCUuuuuUCUUCCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 7106 | 0.67 | 0.989969 |
Target: 5'- aCUCGCGgu-GGCGcucucuuaacagccAGAGGAGAAGGaGGa -3' miRNA: 3'- -GGGCGCaacCCGC--------------UUUUUUCUUCC-CC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 36913 | 0.67 | 0.989837 |
Target: 5'- cCCCGCGcUGGGCcGGGAcguacuccGGGGc -3' miRNA: 3'- -GGGCGCaACCCG-CUUUuuucuu--CCCC- -5' |
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23834 | 3' | -50.7 | NC_005261.1 | + | 5840 | 0.67 | 0.989012 |
Target: 5'- gCCGgGUUacGGCGGccGAGAGAGGGGu -3' miRNA: 3'- gGGCgCAAc-CCGCUu-UUUUCUUCCCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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