Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23868 | 5' | -54.9 | NC_005262.1 | + | 45716 | 0.66 | 0.758424 |
Target: 5'- uUCGaGGCAuUCGAGccAGUucGAGCGCGUCAc -3' miRNA: 3'- uAGC-CUGU-GGUUC--UCG--UUCGCGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 14003 | 0.66 | 0.758424 |
Target: 5'- -cCGGACGgugCAGGGGCGuccGGCGCGgCAg -3' miRNA: 3'- uaGCCUGUg--GUUCUCGU---UCGCGCaGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 58445 | 0.66 | 0.75739 |
Target: 5'- --aGGACGCCcc-GGCAGGCgugcgcgGCGUCAc -3' miRNA: 3'- uagCCUGUGGuucUCGUUCG-------CGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 60677 | 0.66 | 0.748027 |
Target: 5'- cUCGGcaugcuggcgACGCUggGcuucuucGCGGGCGCGUCGg -3' miRNA: 3'- uAGCC----------UGUGGuuCu------CGUUCGCGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 53823 | 0.66 | 0.748027 |
Target: 5'- -aCGGGCAUCGAGcugacGCAccGGCGCGgcgCGg -3' miRNA: 3'- uaGCCUGUGGUUCu----CGU--UCGCGCa--GU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 42285 | 0.66 | 0.737511 |
Target: 5'- -aCGGGCACC---GGCGAcguguucucGCGCGUCu -3' miRNA: 3'- uaGCCUGUGGuucUCGUU---------CGCGCAGu -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 17315 | 0.66 | 0.726888 |
Target: 5'- --aGGAagcagACCGcaaGGAGCGcgAGCGCGUCGc -3' miRNA: 3'- uagCCUg----UGGU---UCUCGU--UCGCGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 56271 | 0.66 | 0.726888 |
Target: 5'- -aCGGACAUCGGGcucGGCGAGCa-GUCGg -3' miRNA: 3'- uaGCCUGUGGUUC---UCGUUCGcgCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 9428 | 0.67 | 0.706449 |
Target: 5'- cGUCGGACgucugccagcccuucGCCGGGuccucguagaucgaGGUgccGAGCGCGUCGa -3' miRNA: 3'- -UAGCCUG---------------UGGUUC--------------UCG---UUCGCGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 7508 | 0.67 | 0.705366 |
Target: 5'- gAUCGcGACGcauCCAucGGGCAgguucggcGGCGCGUCGg -3' miRNA: 3'- -UAGC-CUGU---GGUu-CUCGU--------UCGCGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 21569 | 0.67 | 0.672567 |
Target: 5'- cAUCGGA-ACCGcGAGCGGGUaCGUCGu -3' miRNA: 3'- -UAGCCUgUGGUuCUCGUUCGcGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 15717 | 0.67 | 0.661542 |
Target: 5'- cGUCGG-CGCCGGGcggcucgucgcGGCAGGUGCGa-- -3' miRNA: 3'- -UAGCCuGUGGUUC-----------UCGUUCGCGCagu -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 18594 | 0.67 | 0.650489 |
Target: 5'- -gCGGACAUgccgccugCGAGGGCAGGC-CGUUAc -3' miRNA: 3'- uaGCCUGUG--------GUUCUCGUUCGcGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 11170 | 0.67 | 0.650489 |
Target: 5'- gGUCGcGCGCCAcGAGCcGGUGCGcuUCAg -3' miRNA: 3'- -UAGCcUGUGGUuCUCGuUCGCGC--AGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 47503 | 0.68 | 0.639419 |
Target: 5'- gAUCGGuugcACGCCGcGAGCG-GCGCG-CAg -3' miRNA: 3'- -UAGCC----UGUGGUuCUCGUuCGCGCaGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 9319 | 0.68 | 0.628343 |
Target: 5'- uUCGGcuGCACCGcGAGCA-GCGCcUCGa -3' miRNA: 3'- uAGCC--UGUGGUuCUCGUuCGCGcAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 24786 | 0.68 | 0.621698 |
Target: 5'- -cCGGGCgagcacuucgacaucGCgAAGGGCAAGCGCGa-- -3' miRNA: 3'- uaGCCUG---------------UGgUUCUCGUUCGCGCagu -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 17277 | 0.68 | 0.61727 |
Target: 5'- -cUGGGCugCugcgcGGGCAGGgGCGUCGc -3' miRNA: 3'- uaGCCUGugGuu---CUCGUUCgCGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 37723 | 0.68 | 0.60621 |
Target: 5'- gAUgGGGCGugAAGGGCu-GCGCGUCGa -3' miRNA: 3'- -UAgCCUGUggUUCUCGuuCGCGCAGU- -5' |
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23868 | 5' | -54.9 | NC_005262.1 | + | 52177 | 0.68 | 0.60621 |
Target: 5'- cUC-GACGCCGAGGGCAugaccAGCGUG-CAg -3' miRNA: 3'- uAGcCUGUGGUUCUCGU-----UCGCGCaGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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