Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23876 | 3' | -58.3 | NC_005262.1 | + | 41106 | 0.66 | 0.642678 |
Target: 5'- gCGAgGCGGuCGAGGcgCGuCUGCGcacuGGUCc -3' miRNA: 3'- -GCU-UGCC-GCUCCaaGC-GGCGCu---CCAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 16024 | 0.66 | 0.636327 |
Target: 5'- uCGAgcACGGgGAgcccgcgcgaugccgGGUUCGCCGCGGccGG-Cg -3' miRNA: 3'- -GCU--UGCCgCU---------------CCAAGCGGCGCU--CCaG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 43034 | 0.66 | 0.632092 |
Target: 5'- gCGAucucgUGGCGcugGGGUUCGaCCGggcaGGGGUCg -3' miRNA: 3'- -GCUu----GCCGC---UCCAAGC-GGCg---CUCCAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 34976 | 0.66 | 0.632092 |
Target: 5'- uCGAGCGcgacGCGAGcucgagaugCGCgggCGCGAGGUCa -3' miRNA: 3'- -GCUUGC----CGCUCcaa------GCG---GCGCUCCAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 54842 | 0.66 | 0.632092 |
Target: 5'- gGAugGGUGGaGUgcgUCGCCcgcGCGAGgGUCa -3' miRNA: 3'- gCUugCCGCUcCA---AGCGG---CGCUC-CAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 33806 | 0.66 | 0.621507 |
Target: 5'- gCGGGCaacguccuccaGGaCGAGGaucacaUUCGCUGgGAGGUCc -3' miRNA: 3'- -GCUUG-----------CC-GCUCC------AAGCGGCgCUCCAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 8267 | 0.66 | 0.619391 |
Target: 5'- aGcAGCGGcCGAGGaagcugcccgcgUCGCuCGCGAGG-Cg -3' miRNA: 3'- gC-UUGCC-GCUCCa-----------AGCG-GCGCUCCaG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 10635 | 0.66 | 0.600371 |
Target: 5'- gCGAAgGGCG-GGUugccgaUCGCCGCGucGa- -3' miRNA: 3'- -GCUUgCCGCuCCA------AGCGGCGCucCag -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 186 | 0.66 | 0.600371 |
Target: 5'- uCGGACGGCuucauGGUccaCGCCGgCGGGGg- -3' miRNA: 3'- -GCUUGCCGcu---CCAa--GCGGC-GCUCCag -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 17256 | 0.67 | 0.579334 |
Target: 5'- gGAGCGGCGGccggCGCCGCGcuGGGc- -3' miRNA: 3'- gCUUGCCGCUccaaGCGGCGC--UCCag -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 29362 | 0.67 | 0.579334 |
Target: 5'- gCGAACGGCagaaccuucguGAGGaaUUCGU--CGAGGUCg -3' miRNA: 3'- -GCUUGCCG-----------CUCC--AAGCGgcGCUCCAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 16493 | 0.67 | 0.568873 |
Target: 5'- aCGAGCugGGCGAGGcgcugggCGCUGCGcuGGGg- -3' miRNA: 3'- -GCUUG--CCGCUCCaa-----GCGGCGC--UCCag -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 13397 | 0.67 | 0.558459 |
Target: 5'- --cGCGGCGcuGaUCGCCGCGcGGUUc -3' miRNA: 3'- gcuUGCCGCucCaAGCGGCGCuCCAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 31178 | 0.67 | 0.5481 |
Target: 5'- --uACGGCGuGGUgCGCUGCuGGGUa -3' miRNA: 3'- gcuUGCCGCuCCAaGCGGCGcUCCAg -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 50948 | 0.67 | 0.5481 |
Target: 5'- gCGAAgcuCGGCacGAcGGUUC-CCGCGAGGcCg -3' miRNA: 3'- -GCUU---GCCG--CU-CCAAGcGGCGCUCCaG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 4658 | 0.67 | 0.537802 |
Target: 5'- gCGcGCGGCGAcgcggcccggccGGUUCGCCucgGCGcGGUa -3' miRNA: 3'- -GCuUGCCGCU------------CCAAGCGG---CGCuCCAg -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 43274 | 0.67 | 0.527572 |
Target: 5'- uCGAguGgGGUGAGG-UCGUCGCGAGcGcCg -3' miRNA: 3'- -GCU--UgCCGCUCCaAGCGGCGCUC-CaG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 45215 | 0.67 | 0.527572 |
Target: 5'- uCGAAUGGCuacacgGAGGcccugaacggCGUCGCGaAGGUCa -3' miRNA: 3'- -GCUUGCCG------CUCCaa--------GCGGCGC-UCCAG- -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 42488 | 0.68 | 0.517415 |
Target: 5'- gCGua-GGUGGGGUUggugagcaGCCGCGAGGa- -3' miRNA: 3'- -GCuugCCGCUCCAAg-------CGGCGCUCCag -5' |
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23876 | 3' | -58.3 | NC_005262.1 | + | 40062 | 0.68 | 0.516403 |
Target: 5'- gCGGGCaGGUu-GGUcaaagcagggugaUCGCCGCGuGGUCg -3' miRNA: 3'- -GCUUG-CCGcuCCA-------------AGCGGCGCuCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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