Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24331 | 5' | -58.5 | NC_005264.1 | + | 76693 | 0.66 | 0.83514 |
Target: 5'- uGCCCAGCUugGCgcGGAGCGCgGCg- -3' miRNA: 3'- gUGGGUCGGcaUGa-CCUCGCGaUGgc -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 98472 | 0.66 | 0.83514 |
Target: 5'- aACCCGGCCGgcacGCgcgagGGcaucuccucAGCGCUACa- -3' miRNA: 3'- gUGGGUCGGCa---UGa----CC---------UCGCGAUGgc -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 138774 | 0.66 | 0.818583 |
Target: 5'- gCGCCUGGCCGUGCUGuuG-GCUGuguCCGc -3' miRNA: 3'- -GUGGGUCGGCAUGACcuCgCGAU---GGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 24949 | 0.66 | 0.810057 |
Target: 5'- gCACaUguGCCGUACcaGGGCGCUgcGCCGa -3' miRNA: 3'- -GUG-GguCGGCAUGacCUCGCGA--UGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 157569 | 0.66 | 0.810057 |
Target: 5'- aCGCCacgaGGCCGgGCUuGGGCGCUuccCCGu -3' miRNA: 3'- -GUGGg---UCGGCaUGAcCUCGCGAu--GGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 66067 | 0.66 | 0.810057 |
Target: 5'- aGCCCGGgaauaaguuucUCGUAgucuCUGGGGgGCUGCCc -3' miRNA: 3'- gUGGGUC-----------GGCAU----GACCUCgCGAUGGc -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 44631 | 0.67 | 0.792546 |
Target: 5'- uCACCgCGGCCGag--GGAGUGCUgauguagaagcuGCCGu -3' miRNA: 3'- -GUGG-GUCGGCaugaCCUCGCGA------------UGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 55135 | 0.67 | 0.792546 |
Target: 5'- aCACCCcGCCGUGCgcu-GUGCUGgCGc -3' miRNA: 3'- -GUGGGuCGGCAUGaccuCGCGAUgGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 77970 | 0.67 | 0.792546 |
Target: 5'- cCugCCGGCgccgcgCGUACcGGAGCGggcgaUACCGa -3' miRNA: 3'- -GugGGUCG------GCAUGaCCUCGCg----AUGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 99771 | 0.67 | 0.783578 |
Target: 5'- gGCCCucGCCGaGCUGGAGaccgagaucaaGCgGCCGa -3' miRNA: 3'- gUGGGu-CGGCaUGACCUCg----------CGaUGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 104939 | 0.67 | 0.783578 |
Target: 5'- -cCCCAGCCGUGCca-AGCGCcgaGCCu -3' miRNA: 3'- guGGGUCGGCAUGaccUCGCGa--UGGc -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 70385 | 0.67 | 0.783578 |
Target: 5'- gGCUCGGUgGaGCUGGAGgGCgGCCu -3' miRNA: 3'- gUGGGUCGgCaUGACCUCgCGaUGGc -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 98980 | 0.67 | 0.746485 |
Target: 5'- ---aCGGCCGUugUGGAGCG--GCCu -3' miRNA: 3'- guggGUCGGCAugACCUCGCgaUGGc -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 799 | 0.68 | 0.736949 |
Target: 5'- gCGCuCCAGUacgGCUGGGuGCGCgGCCGg -3' miRNA: 3'- -GUG-GGUCGgcaUGACCU-CGCGaUGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 12531 | 0.68 | 0.727325 |
Target: 5'- gCAUCUAGCCGguaggaGCggGGGGCGUUcGCCGc -3' miRNA: 3'- -GUGGGUCGGCa-----UGa-CCUCGCGA-UGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 131558 | 0.68 | 0.727325 |
Target: 5'- gCAUCUAGCCGguaggaGCggGGGGCGUUcGCCGc -3' miRNA: 3'- -GUGGGUCGGCa-----UGa-CCUCGCGA-UGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 98669 | 0.68 | 0.717623 |
Target: 5'- gACCCAGU---GCUGGcGGCGCUugCa -3' miRNA: 3'- gUGGGUCGgcaUGACC-UCGCGAugGc -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 52425 | 0.69 | 0.678196 |
Target: 5'- gACCUGGCCGagaagUACUGGcucgAGC-CUGCCGa -3' miRNA: 3'- gUGGGUCGGC-----AUGACC----UCGcGAUGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 95434 | 0.69 | 0.648214 |
Target: 5'- aACCCAaaGCCGUcgUGGAauaCGCUACCGc -3' miRNA: 3'- gUGGGU--CGGCAugACCUc--GCGAUGGC- -5' |
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24331 | 5' | -58.5 | NC_005264.1 | + | 48377 | 0.7 | 0.588118 |
Target: 5'- gGCgCAGCCGguagACUGGGGCGUcgGgCGg -3' miRNA: 3'- gUGgGUCGGCa---UGACCUCGCGa-UgGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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