Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24806 | 3' | -53.5 | NC_005284.1 | + | 43851 | 0.66 | 0.782327 |
Target: 5'- gGCGaCGAGgccgGC-CAUGCgGGCcuGCGCAu -3' miRNA: 3'- -UGC-GUUCaa--CGuGUAUGgCCG--UGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 4419 | 0.66 | 0.782327 |
Target: 5'- -aGCGGGUUaagacgGCACAcacCCGGCacACGCAg -3' miRNA: 3'- ugCGUUCAA------CGUGUau-GGCCG--UGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 601 | 0.66 | 0.772065 |
Target: 5'- -gGCAAGc-GCccuGCGUGCCGGUAUGUg -3' miRNA: 3'- ugCGUUCaaCG---UGUAUGGCCGUGCGu -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 36302 | 0.66 | 0.772065 |
Target: 5'- cACGCcga--GCGCGUucUCGGCGCGCAc -3' miRNA: 3'- -UGCGuucaaCGUGUAu-GGCCGUGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 33584 | 0.66 | 0.772065 |
Target: 5'- -aGCcGGgcgUGCACGUcaggACCGGCucCGCGu -3' miRNA: 3'- ugCGuUCa--ACGUGUA----UGGCCGu-GCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 28448 | 0.66 | 0.761651 |
Target: 5'- gACGCAGGUcUGC-C--ACCGGCACauuGCGc -3' miRNA: 3'- -UGCGUUCA-ACGuGuaUGGCCGUG---CGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 27647 | 0.66 | 0.758499 |
Target: 5'- cCGC-AGUUGCAUcgcagaugcgucgcgGCgGGCACGCGc -3' miRNA: 3'- uGCGuUCAACGUGua-------------UGgCCGUGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 32326 | 0.66 | 0.751098 |
Target: 5'- uUGCAGGUaGUagaaGCGUGCCG-CACGCu -3' miRNA: 3'- uGCGUUCAaCG----UGUAUGGCcGUGCGu -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 8905 | 0.67 | 0.729621 |
Target: 5'- -gGCAAGUUGCGCGgcaGCaguGGCuuCGCGc -3' miRNA: 3'- ugCGUUCAACGUGUa--UGg--CCGu-GCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 16146 | 0.67 | 0.718723 |
Target: 5'- cGCGCAGccGUgggGCACAUcgcugaugggGCuCGGCGCGUc -3' miRNA: 3'- -UGCGUU--CAa--CGUGUA----------UG-GCCGUGCGu -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 40832 | 0.67 | 0.707735 |
Target: 5'- uUGCG---UGCGCGU-CCGGCACuGCAa -3' miRNA: 3'- uGCGUucaACGUGUAuGGCCGUG-CGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 12332 | 0.67 | 0.707735 |
Target: 5'- aACGCAGGgagGCGau--CCGGCuucGCGCGa -3' miRNA: 3'- -UGCGUUCaa-CGUguauGGCCG---UGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 35649 | 0.67 | 0.707735 |
Target: 5'- uGCGuCAGGUU-CACgAUGCCGuucGCGCGCGg -3' miRNA: 3'- -UGC-GUUCAAcGUG-UAUGGC---CGUGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 33183 | 0.67 | 0.707735 |
Target: 5'- cGCGCGAGaucUUGCccaacgcuucgGCAUGCCGcuGCGCGUc -3' miRNA: 3'- -UGCGUUC---AACG-----------UGUAUGGC--CGUGCGu -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 21887 | 0.67 | 0.693336 |
Target: 5'- gACGCAgcGGUUGCggaacgcguuuucgGCGUcGCCGGCgacgaucaGCGCGa -3' miRNA: 3'- -UGCGU--UCAACG--------------UGUA-UGGCCG--------UGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 17684 | 0.68 | 0.689997 |
Target: 5'- cGCGCGAucgUGCGCGacgcccugaaucucGCCGGCGCGUc -3' miRNA: 3'- -UGCGUUca-ACGUGUa-------------UGGCCGUGCGu -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 49113 | 0.68 | 0.685537 |
Target: 5'- aGCGCGAGggcgaagUGUuCAUGCguuuuaaGGCGCGCGu -3' miRNA: 3'- -UGCGUUCa------ACGuGUAUGg------CCGUGCGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 19303 | 0.68 | 0.685537 |
Target: 5'- gACGCAAGUacaugGCAUcgAUCGGCGuggGCAu -3' miRNA: 3'- -UGCGUUCAa----CGUGuaUGGCCGUg--CGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 42026 | 0.68 | 0.663124 |
Target: 5'- uCGCGA---GCGCGUGCCGGCcgaGCGg -3' miRNA: 3'- uGCGUUcaaCGUGUAUGGCCGug-CGU- -5' |
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24806 | 3' | -53.5 | NC_005284.1 | + | 45507 | 0.68 | 0.663124 |
Target: 5'- cACGCGAuGUUGUACcgAUgGGaCGCGUAa -3' miRNA: 3'- -UGCGUU-CAACGUGuaUGgCC-GUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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