Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24813 | 5' | -53.1 | NC_005284.1 | + | 8434 | 0.66 | 0.869395 |
Target: 5'- gCCGAGAuggucCGGCGagCC-CGCGAcggcggagggagaggAAGGCg -3' miRNA: 3'- gGGCUCU-----GUUGCa-GGaGCGCU---------------UUCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 21256 | 0.66 | 0.866201 |
Target: 5'- gCUCGaAGAUGA--UCUUCGCGAAGcGGCa -3' miRNA: 3'- -GGGC-UCUGUUgcAGGAGCGCUUU-CCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 11639 | 0.66 | 0.865396 |
Target: 5'- aCUCGGGGCGAUGUCgaugggugacaaCgcgCGCGAGcugaaucAGGCc -3' miRNA: 3'- -GGGCUCUGUUGCAG------------Ga--GCGCUU-------UCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 50941 | 0.66 | 0.858047 |
Target: 5'- uUCCGcGAUAcGCGaUCCUUGCGuucGGCg -3' miRNA: 3'- -GGGCuCUGU-UGC-AGGAGCGCuuuCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 48328 | 0.66 | 0.858047 |
Target: 5'- aUCCGgaAGGCGGCGgcgagaugaUUCUCGCGcu-GGCa -3' miRNA: 3'- -GGGC--UCUGUUGC---------AGGAGCGCuuuCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 24335 | 0.66 | 0.858047 |
Target: 5'- -gUGGGGCAACG-CUuaUCGCGucgcAAGGCu -3' miRNA: 3'- ggGCUCUGUUGCaGG--AGCGCu---UUCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 17376 | 0.66 | 0.849658 |
Target: 5'- uCCCGGccGGCuACGUCggUGUGAAGGuGCa -3' miRNA: 3'- -GGGCU--CUGuUGCAGgaGCGCUUUC-CG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 25074 | 0.66 | 0.849658 |
Target: 5'- gCCUGAuGACGcCGgccgaCCUCGCGAucgucaccGGCa -3' miRNA: 3'- -GGGCU-CUGUuGCa----GGAGCGCUuu------CCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 9850 | 0.66 | 0.849658 |
Target: 5'- gCCCuacGCGGCGaucaUCgCUCGCGAGAcGGCa -3' miRNA: 3'- -GGGcucUGUUGC----AG-GAGCGCUUU-CCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 36092 | 0.66 | 0.841042 |
Target: 5'- gUCgGAGAacuuggucGCGUCgUCGCGAuucGGCg -3' miRNA: 3'- -GGgCUCUgu------UGCAGgAGCGCUuu-CCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 54287 | 0.67 | 0.832208 |
Target: 5'- gCCGAG-CAGC---UUCGCGAAAGGg -3' miRNA: 3'- gGGCUCuGUUGcagGAGCGCUUUCCg -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 52988 | 0.67 | 0.823164 |
Target: 5'- gCCGAGGCAA-----UCGCGguGGGCa -3' miRNA: 3'- gGGCUCUGUUgcaggAGCGCuuUCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 45615 | 0.67 | 0.81392 |
Target: 5'- gCCG-GACGAgggcggugcgaGUCUUCGCGGGgcAGGCu -3' miRNA: 3'- gGGCuCUGUUg----------CAGGAGCGCUU--UCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 10006 | 0.67 | 0.804486 |
Target: 5'- gCCGAGACGgaccGCGgCCUUGCGcAAGccgaGCg -3' miRNA: 3'- gGGCUCUGU----UGCaGGAGCGCuUUC----CG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 39213 | 0.67 | 0.794872 |
Target: 5'- gUCGAGAaagcCAGCGUCCUgauaGUGGuuGAGGUa -3' miRNA: 3'- gGGCUCU----GUUGCAGGAg---CGCU--UUCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 35118 | 0.67 | 0.794872 |
Target: 5'- gCCCGAuaGGCGGCGUgUUCuGCGGAuGuGCg -3' miRNA: 3'- -GGGCU--CUGUUGCAgGAG-CGCUUuC-CG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 47256 | 0.67 | 0.794872 |
Target: 5'- aCCCgGAGACgGGCGagaaCCUCGUGAucgacGGCc -3' miRNA: 3'- -GGG-CUCUG-UUGCa---GGAGCGCUuu---CCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 54384 | 0.67 | 0.794872 |
Target: 5'- aCCGGGGguGCGgugcgcUCgUCGCGAAuGGUa -3' miRNA: 3'- gGGCUCUguUGC------AGgAGCGCUUuCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 30793 | 0.67 | 0.793902 |
Target: 5'- aCCGAcGAUAGuccggauCGUCCcgaUCGCGGucguAGGCg -3' miRNA: 3'- gGGCU-CUGUU-------GCAGG---AGCGCUu---UCCG- -5' |
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24813 | 5' | -53.1 | NC_005284.1 | + | 43983 | 0.67 | 0.78509 |
Target: 5'- uCgCGGGAUGACGcCCUCGgGuucaaugaaguaAGGGGCg -3' miRNA: 3'- -GgGCUCUGUUGCaGGAGCgC------------UUUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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