Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24814 | 5' | -55.8 | NC_005284.1 | + | 47255 | 0.66 | 0.720869 |
Target: 5'- gACCCGGagA-CGGGCGagaaccucgugaucgACGGccgUCGCCg -3' miRNA: 3'- -UGGGUUagUgGCCCGU---------------UGCCa--AGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 27652 | 0.66 | 0.716633 |
Target: 5'- aACCucgggCAAUCGCCGcuauacgggccGGCGuggcgcGCGGgUCGCCg -3' miRNA: 3'- -UGG-----GUUAGUGGC-----------CCGU------UGCCaAGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 11317 | 0.66 | 0.716633 |
Target: 5'- uGCUCGAcaaCAUCcGGCAGCGGaUCGCg -3' miRNA: 3'- -UGGGUUa--GUGGcCCGUUGCCaAGCGg -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 15361 | 0.66 | 0.713448 |
Target: 5'- gGCUgCAAg-ACCGGGCGgaagaacgucggaaACGG-UCGCCa -3' miRNA: 3'- -UGG-GUUagUGGCCCGU--------------UGCCaAGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 21235 | 0.66 | 0.695271 |
Target: 5'- gGCgCAcauAUCAgcUCGcGGCGGCGGUgagcuUCGCCu -3' miRNA: 3'- -UGgGU---UAGU--GGC-CCGUUGCCA-----AGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 48433 | 0.66 | 0.694196 |
Target: 5'- cGCgCCAAUCGCCGcGuGCAguGCaGUaccagcuUCGCCg -3' miRNA: 3'- -UG-GGUUAGUGGC-C-CGU--UGcCA-------AGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 44704 | 0.66 | 0.684494 |
Target: 5'- gACCC----GCCGcGGUcGCGG-UCGCCg -3' miRNA: 3'- -UGGGuuagUGGC-CCGuUGCCaAGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 23885 | 0.66 | 0.673668 |
Target: 5'- cGCCCGAugcaUCccgACUGGGCu-CGGU-CGCUg -3' miRNA: 3'- -UGGGUU----AG---UGGCCCGuuGCCAaGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 13573 | 0.67 | 0.662804 |
Target: 5'- -gCCAGUUgcagcaguACUGGGUAGCuGUUCGCa -3' miRNA: 3'- ugGGUUAG--------UGGCCCGUUGcCAAGCGg -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 21633 | 0.67 | 0.662804 |
Target: 5'- cGCUCGA-CGCCGaGCAGCGuGcgaUCGCCg -3' miRNA: 3'- -UGGGUUaGUGGCcCGUUGC-Ca--AGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 52856 | 0.67 | 0.651911 |
Target: 5'- aGCCCuucGUCcucgaGCUGGuGCGACaGGUUCGUUg -3' miRNA: 3'- -UGGGu--UAG-----UGGCC-CGUUG-CCAAGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 45270 | 0.67 | 0.64973 |
Target: 5'- cCUCGAcgagcgcUCGCCGGGCGccgacccACGcugUCGCCg -3' miRNA: 3'- uGGGUU-------AGUGGCCCGU-------UGCca-AGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 27086 | 0.67 | 0.641 |
Target: 5'- aAUCCAucgCGCCGGaaucagGCAGCGGcgacccgcgCGCCa -3' miRNA: 3'- -UGGGUua-GUGGCC------CGUUGCCaa-------GCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 44557 | 0.67 | 0.630082 |
Target: 5'- cCCCAAgcgcCACCGuGCccACGG-UCGCCa -3' miRNA: 3'- uGGGUUa---GUGGCcCGu-UGCCaAGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 23349 | 0.67 | 0.630082 |
Target: 5'- gGCCCGGcgCGCC--GCAGCGGcgcacgUCGCCc -3' miRNA: 3'- -UGGGUUa-GUGGccCGUUGCCa-----AGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 8211 | 0.68 | 0.597374 |
Target: 5'- uGCCCAcugCGCCGccaugcucGGCAAcauCGGaUCGCCc -3' miRNA: 3'- -UGGGUua-GUGGC--------CCGUU---GCCaAGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 24639 | 0.68 | 0.585434 |
Target: 5'- gACUCAGggGCCGGGCGaagGCGGUguuuuaugaguggUCGUg -3' miRNA: 3'- -UGGGUUagUGGCCCGU---UGCCA-------------AGCGg -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 4438 | 0.68 | 0.564926 |
Target: 5'- cACCCGG-CACac-GCAgaaagcACGGUUCGCCg -3' miRNA: 3'- -UGGGUUaGUGgccCGU------UGCCAAGCGG- -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 7912 | 0.68 | 0.564926 |
Target: 5'- gUUCAAUCGCCGGcGCAAgguccgguCGGUUgGCg -3' miRNA: 3'- uGGGUUAGUGGCC-CGUU--------GCCAAgCGg -5' |
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24814 | 5' | -55.8 | NC_005284.1 | + | 5899 | 0.68 | 0.564926 |
Target: 5'- cACCUucgcCGCCGGGCGuguccACGG--CGCCg -3' miRNA: 3'- -UGGGuua-GUGGCCCGU-----UGCCaaGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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