Results 1 - 20 of 51 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24825 | 3' | -52.1 | NC_005284.1 | + | 31272 | 0.66 | 0.906707 |
Target: 5'- gCGUGUCGGcgaUCAGGCg-GAacagGUcCGCGCg -3' miRNA: 3'- -GUACAGCU---AGUCUGagCUg---CA-GCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 46684 | 0.66 | 0.906707 |
Target: 5'- -----aGGUCGGGCUCGAguCGgaaaCGCGCc -3' miRNA: 3'- guacagCUAGUCUGAGCU--GCa---GCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 23081 | 0.66 | 0.906707 |
Target: 5'- gUAUGUCGAUgcGGCU--ACGUaCGCGCu -3' miRNA: 3'- -GUACAGCUAguCUGAgcUGCA-GCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 45412 | 0.66 | 0.906707 |
Target: 5'- aGUGUCGcaGUCGcggccggcGACgauauucgCGGCGUCGCGa -3' miRNA: 3'- gUACAGC--UAGU--------CUGa-------GCUGCAGCGCg -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 7667 | 0.66 | 0.906707 |
Target: 5'- --cGUCGGUCaauGGGCUgauuucggCGACGUacaGCGUg -3' miRNA: 3'- guaCAGCUAG---UCUGA--------GCUGCAg--CGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 10042 | 0.66 | 0.90469 |
Target: 5'- gAUGUCGcgcuugccacgCAGGCUCGugagcgaGCGgaaCGCGCg -3' miRNA: 3'- gUACAGCua---------GUCUGAGC-------UGCa--GCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 43435 | 0.66 | 0.899887 |
Target: 5'- -uUGUCGGUUGcGACcgccUCGACGcgcagUUGCGCg -3' miRNA: 3'- guACAGCUAGU-CUG----AGCUGC-----AGCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 44571 | 0.66 | 0.899887 |
Target: 5'- uGUGUCGAUCacguccuggcGGACaaCGACGUUGUa- -3' miRNA: 3'- gUACAGCUAG----------UCUGa-GCUGCAGCGcg -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 11246 | 0.66 | 0.892794 |
Target: 5'- uGUGUCGAcCAacgcGGCagCGGC-UCGCGCg -3' miRNA: 3'- gUACAGCUaGU----CUGa-GCUGcAGCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 45157 | 0.66 | 0.892794 |
Target: 5'- --gGUCGA-CGcACggCGACGUCGgGCa -3' miRNA: 3'- guaCAGCUaGUcUGa-GCUGCAGCgCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 9199 | 0.66 | 0.892794 |
Target: 5'- gCGUGUaugUGAUCAGGCgUCGAUagccgagUGCGCg -3' miRNA: 3'- -GUACA---GCUAGUCUG-AGCUGca-----GCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 33613 | 0.66 | 0.892794 |
Target: 5'- gCGUGcUCGAUgAGccguuGCUCGAgcCG-CGCGCg -3' miRNA: 3'- -GUAC-AGCUAgUC-----UGAGCU--GCaGCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 9291 | 0.66 | 0.885433 |
Target: 5'- --cGaCGAUCGGGCagaCGAUGaCGCGCc -3' miRNA: 3'- guaCaGCUAGUCUGa--GCUGCaGCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 37092 | 0.66 | 0.885433 |
Target: 5'- --cGcCGGcgAGAUUCagGGCGUCGCGCa -3' miRNA: 3'- guaCaGCUagUCUGAG--CUGCAGCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 36640 | 0.66 | 0.877807 |
Target: 5'- uCGUGUagaCGAUCuGGC-CGuCGUCGuCGCu -3' miRNA: 3'- -GUACA---GCUAGuCUGaGCuGCAGC-GCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 51199 | 0.66 | 0.877807 |
Target: 5'- --cGUCGA-CuGACUUGACGUCGa-- -3' miRNA: 3'- guaCAGCUaGuCUGAGCUGCAGCgcg -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 49262 | 0.66 | 0.87703 |
Target: 5'- --cG-CGAUCAGGaggCGACGUgacgaagCGCGCu -3' miRNA: 3'- guaCaGCUAGUCUga-GCUGCA-------GCGCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 7306 | 0.67 | 0.853409 |
Target: 5'- ---cUCGGUguGGC-CGACGUCGaGCa -3' miRNA: 3'- guacAGCUAguCUGaGCUGCAGCgCG- -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 42015 | 0.67 | 0.853409 |
Target: 5'- gCAUGcCGAUCgAGGaaaUCGAUGaUCGCGg -3' miRNA: 3'- -GUACaGCUAG-UCUg--AGCUGC-AGCGCg -5' |
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24825 | 3' | -52.1 | NC_005284.1 | + | 6922 | 0.67 | 0.853409 |
Target: 5'- gGUGgugCGAagGGGuauCUCGGCGUCGCa- -3' miRNA: 3'- gUACa--GCUagUCU---GAGCUGCAGCGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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