Results 1 - 20 of 80 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25507 | 3' | -54.4 | NC_005337.1 | + | 75753 | 0.65 | 0.947618 |
Target: 5'- aCGUCACcaGCAUCGagggcgugauuccGCCGCucguuaaGCuGCUGGa -3' miRNA: 3'- -GUAGUGaaCGUAGC-------------UGGCG-------CG-CGACC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 36211 | 0.66 | 0.944048 |
Target: 5'- uCGUCGuuuUUUGCGcCGGCCGCgGCGCg-- -3' miRNA: 3'- -GUAGU---GAACGUaGCUGGCG-CGCGacc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 8143 | 0.66 | 0.944048 |
Target: 5'- --aCGCcggGCAU-GGCCGCGCGCa-- -3' miRNA: 3'- guaGUGaa-CGUAgCUGGCGCGCGacc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 94256 | 0.66 | 0.944048 |
Target: 5'- --cCGCUaGCAUCuccACCGCGCGgaUGGc -3' miRNA: 3'- guaGUGAaCGUAGc--UGGCGCGCg-ACC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 105353 | 0.66 | 0.944048 |
Target: 5'- ---gGCUccCGUCGAUCGCGUGCUuGGu -3' miRNA: 3'- guagUGAacGUAGCUGGCGCGCGA-CC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 126436 | 0.66 | 0.944048 |
Target: 5'- --gCGCgcgUGCG-CGuguaccACCGCGCGCUGu -3' miRNA: 3'- guaGUGa--ACGUaGC------UGGCGCGCGACc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 88932 | 0.66 | 0.944048 |
Target: 5'- cCGUCGucUGCGagcUCgcgGACCGCGCGgaGGc -3' miRNA: 3'- -GUAGUgaACGU---AG---CUGGCGCGCgaCC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 78080 | 0.66 | 0.943591 |
Target: 5'- -cUC-CUUGCGguaGAgcuuguuCCGCGCGCUGa -3' miRNA: 3'- guAGuGAACGUag-CU-------GGCGCGCGACc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 67956 | 0.66 | 0.939369 |
Target: 5'- uGUCGCcaGCccgCGACCGCgGCGCUc- -3' miRNA: 3'- gUAGUGaaCGua-GCUGGCG-CGCGAcc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 100393 | 0.66 | 0.939369 |
Target: 5'- gCAUCGagUGCG-CGGgCGCGCGCUa- -3' miRNA: 3'- -GUAGUgaACGUaGCUgGCGCGCGAcc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 123928 | 0.66 | 0.934444 |
Target: 5'- --cCGCUgcgGCGgcgaCGGCCGCGUGCa-- -3' miRNA: 3'- guaGUGAa--CGUa---GCUGGCGCGCGacc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 47996 | 0.66 | 0.934444 |
Target: 5'- gCcgCACUgcgugGCcUgGAuCCGCGCGCUGcGg -3' miRNA: 3'- -GuaGUGAa----CGuAgCU-GGCGCGCGAC-C- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 50761 | 0.66 | 0.929273 |
Target: 5'- --aCGCgcGCAUCGACCuCGCGCc-- -3' miRNA: 3'- guaGUGaaCGUAGCUGGcGCGCGacc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 14411 | 0.66 | 0.929273 |
Target: 5'- gGUUguGCUUGCcgCGcgacguGCCGC-CGCUGGc -3' miRNA: 3'- gUAG--UGAACGuaGC------UGGCGcGCGACC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 124423 | 0.66 | 0.923857 |
Target: 5'- gGUC-CUggGCAaCG-CCGCGCGCgGGu -3' miRNA: 3'- gUAGuGAa-CGUaGCuGGCGCGCGaCC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 108747 | 0.66 | 0.923857 |
Target: 5'- -cUCGCggcGCGUgGGCCGCuGC-CUGGa -3' miRNA: 3'- guAGUGaa-CGUAgCUGGCG-CGcGACC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 23908 | 0.66 | 0.923857 |
Target: 5'- uGUCGCUgccGCG-CGcCCGCGCGCc-- -3' miRNA: 3'- gUAGUGAa--CGUaGCuGGCGCGCGacc -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 93822 | 0.66 | 0.923857 |
Target: 5'- uCGUCugcgGCGUCGucaaggGCCGUGCGCUccGGa -3' miRNA: 3'- -GUAGugaaCGUAGC------UGGCGCGCGA--CC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 131904 | 0.66 | 0.923857 |
Target: 5'- --gCACguggacGCgGUCGACCggugcugcgGCGCGCUGGc -3' miRNA: 3'- guaGUGaa----CG-UAGCUGG---------CGCGCGACC- -5' |
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25507 | 3' | -54.4 | NC_005337.1 | + | 82173 | 0.66 | 0.923857 |
Target: 5'- cCGUCGCgcaccUGCAcgCGGCCGCcggGCGCgcacGGc -3' miRNA: 3'- -GUAGUGa----ACGUa-GCUGGCG---CGCGa---CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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