Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25517 | 5' | -55.1 | NC_005337.1 | + | 59164 | 0.66 | 0.921121 |
Target: 5'- -aCGACGuuccUCCCCCAGGCCgugcgUCGgggcACCg -3' miRNA: 3'- gaGUUGU----AGGGGGUCUGGa----GGU----UGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 53828 | 0.66 | 0.915325 |
Target: 5'- -aCAGCGUCuCCUCGGACCggCUGGCgCUg -3' miRNA: 3'- gaGUUGUAG-GGGGUCUGGa-GGUUG-GA- -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 133267 | 0.66 | 0.915325 |
Target: 5'- uUCGACggCCUCCAGGcgacgcccgcCCUCCGccgcGCCg -3' miRNA: 3'- gAGUUGuaGGGGGUCU----------GGAGGU----UGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 133267 | 0.66 | 0.915325 |
Target: 5'- uUCGACggCCUCCAGGcgacgcccgcCCUCCGccgcGCCg -3' miRNA: 3'- gAGUUGuaGGGGGUCU----------GGAGGU----UGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 53746 | 0.66 | 0.913538 |
Target: 5'- gUgGACAUCuccgcgCCCCGGGCCUcggcgugcccgcccCCGGCCg -3' miRNA: 3'- gAgUUGUAG------GGGGUCUGGA--------------GGUUGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 56603 | 0.66 | 0.909282 |
Target: 5'- gUCGACcgCUUCCuGGCCUucgCCGGCCg -3' miRNA: 3'- gAGUUGuaGGGGGuCUGGA---GGUUGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 32753 | 0.66 | 0.909282 |
Target: 5'- gUC-ACGUCCa-CGG-CCUCCAGCCg -3' miRNA: 3'- gAGuUGUAGGggGUCuGGAGGUUGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 102249 | 0.66 | 0.909282 |
Target: 5'- uUCGugAUCUCCUccgcgaAGACCUUCAucACCg -3' miRNA: 3'- gAGUugUAGGGGG------UCUGGAGGU--UGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 123548 | 0.66 | 0.89713 |
Target: 5'- cCUCGACAUCCUCCGGcGCCggucggaggacgagcCCGAgCUc -3' miRNA: 3'- -GAGUUGUAGGGGGUC-UGGa--------------GGUUgGA- -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 124679 | 0.66 | 0.896467 |
Target: 5'- gUCAGgAUCCCCguGcUCUUCGACCc -3' miRNA: 3'- gAGUUgUAGGGGguCuGGAGGUUGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 112739 | 0.66 | 0.896467 |
Target: 5'- uUCAACGUCgugaaCCCCuccGGCUuucgUCCGGCCUg -3' miRNA: 3'- gAGUUGUAG-----GGGGu--CUGG----AGGUUGGA- -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 29496 | 0.66 | 0.889699 |
Target: 5'- uUCAGCcggaaGUCCUCCAGGagcuUCUCCAGCa- -3' miRNA: 3'- gAGUUG-----UAGGGGGUCU----GGAGGUUGga -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 123935 | 0.66 | 0.889699 |
Target: 5'- -aCGACGUUCCCCAcGCC-CCGcgaGCCg -3' miRNA: 3'- gaGUUGUAGGGGGUcUGGaGGU---UGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 45975 | 0.67 | 0.882697 |
Target: 5'- --uGACGUCCCCgAGcuugauguccGCCUCC-ACCg -3' miRNA: 3'- gagUUGUAGGGGgUC----------UGGAGGuUGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 72057 | 0.67 | 0.875464 |
Target: 5'- aUCGGCAUCCCCguGuCCcgCCGgaaacugaACCUg -3' miRNA: 3'- gAGUUGUAGGGGguCuGGa-GGU--------UGGA- -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 1773 | 0.67 | 0.868007 |
Target: 5'- -gCGGCAUCCCgccggccgCCAGucCCUCCuGCCg -3' miRNA: 3'- gaGUUGUAGGG--------GGUCu-GGAGGuUGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 121546 | 0.67 | 0.852438 |
Target: 5'- --gGACAUCCUCCGGcACCUCCucgAGCg- -3' miRNA: 3'- gagUUGUAGGGGGUC-UGGAGG---UUGga -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 89085 | 0.67 | 0.852438 |
Target: 5'- -gCGACAgCgCCCCGGGCacggCCAGCCg -3' miRNA: 3'- gaGUUGUaG-GGGGUCUGga--GGUUGGa -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 85426 | 0.68 | 0.844341 |
Target: 5'- -gCAGCAUCUCCCGGuacuCCUUgAGCUUc -3' miRNA: 3'- gaGUUGUAGGGGGUCu---GGAGgUUGGA- -5' |
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25517 | 5' | -55.1 | NC_005337.1 | + | 33752 | 0.68 | 0.844341 |
Target: 5'- --aGACAUCCCCC--ACCUCCGcGCUg -3' miRNA: 3'- gagUUGUAGGGGGucUGGAGGU-UGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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