Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26026 | 3' | -53.6 | NC_005342.2 | + | 9263 | 0.66 | 0.820475 |
Target: 5'- gCUUCACGGUCGgguuuucacCGCcGGCCGg -3' miRNA: 3'- -GAAGUGCCAGCacugcuu--GCG-CUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 2570 | 0.66 | 0.819549 |
Target: 5'- ---gGCGGUCGUGAaGGucgcccaauuGCGCGACa- -3' miRNA: 3'- gaagUGCCAGCACUgCU----------UGCGCUGgc -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 8955 | 0.66 | 0.819549 |
Target: 5'- ---uGCGGcCGUGcGCGGcgGCGACCGa -3' miRNA: 3'- gaagUGCCaGCAC-UGCUugCGCUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 20457 | 0.66 | 0.816759 |
Target: 5'- --gCGcCGGUCGUGACGcAGCcgguuagcgucccgGCGGCUGc -3' miRNA: 3'- gaaGU-GCCAGCACUGC-UUG--------------CGCUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 35713 | 0.66 | 0.81018 |
Target: 5'- gUUCGCGGUCGccuUGAugGgGAUCa -3' miRNA: 3'- gAAGUGCCAGCacuGCUugCgCUGGc -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 22405 | 0.66 | 0.81018 |
Target: 5'- gCUUCGCGG-CGc-AUGAGCGC-ACCGa -3' miRNA: 3'- -GAAGUGCCaGCacUGCUUGCGcUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 13328 | 0.66 | 0.790889 |
Target: 5'- --gCACGGU-GU-ACGGGCGCGGCaCGa -3' miRNA: 3'- gaaGUGCCAgCAcUGCUUGCGCUG-GC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 24645 | 0.66 | 0.780989 |
Target: 5'- -aUCACGGUCGc-AgGGACGuUGACCa -3' miRNA: 3'- gaAGUGCCAGCacUgCUUGC-GCUGGc -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 27524 | 0.67 | 0.770933 |
Target: 5'- gCUUCGCGccgccgaacguGUUuucgaaggcgGUGACGAgcgGCGCGGCCa -3' miRNA: 3'- -GAAGUGC-----------CAG----------CACUGCU---UGCGCUGGc -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 8488 | 0.67 | 0.770933 |
Target: 5'- gUUCGCGaagaaGUCGcccgUGcCGAacaGCGCGACCGg -3' miRNA: 3'- gAAGUGC-----CAGC----ACuGCU---UGCGCUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 20212 | 0.67 | 0.770933 |
Target: 5'- -cUCGCGcuuUCGUGGCGGcgaucgacCGCGACUGg -3' miRNA: 3'- gaAGUGCc--AGCACUGCUu-------GCGCUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 4664 | 0.67 | 0.760733 |
Target: 5'- -cUCGCGuUCGaGGCGAgcGCGCGACaCGc -3' miRNA: 3'- gaAGUGCcAGCaCUGCU--UGCGCUG-GC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 22857 | 0.67 | 0.750401 |
Target: 5'- -gUCGCGG-CccGGCGAucgGCGCGAUCGu -3' miRNA: 3'- gaAGUGCCaGcaCUGCU---UGCGCUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 2104 | 0.67 | 0.749361 |
Target: 5'- -aUCACGG-CGUGAuccucgaacuugcCGuAGCGCGcACCGc -3' miRNA: 3'- gaAGUGCCaGCACU-------------GC-UUGCGC-UGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 19731 | 0.67 | 0.738896 |
Target: 5'- -gUCACGGUcacCGUGACGcgcccgGACGCGcaaggcaACCa -3' miRNA: 3'- gaAGUGCCA---GCACUGC------UUGCGC-------UGGc -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 27735 | 0.67 | 0.729385 |
Target: 5'- -gUCGCGGUCGaucgccgccACGAaaGCGCGAgCCGc -3' miRNA: 3'- gaAGUGCCAGCac-------UGCU--UGCGCU-GGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 28495 | 0.67 | 0.729385 |
Target: 5'- --gCGCGGgUCGcGGCG-GCGCGGCUGc -3' miRNA: 3'- gaaGUGCC-AGCaCUGCuUGCGCUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 38057 | 0.68 | 0.718725 |
Target: 5'- --gCGCGGcgaccugcUCGUGugGGacggucACGCGAUCGa -3' miRNA: 3'- gaaGUGCC--------AGCACugCU------UGCGCUGGC- -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 10697 | 0.68 | 0.707978 |
Target: 5'- aUUgACGGUCGUuuucccgacuGGCGGcguaucguaccGCGCGGCCc -3' miRNA: 3'- gAAgUGCCAGCA----------CUGCU-----------UGCGCUGGc -5' |
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26026 | 3' | -53.6 | NC_005342.2 | + | 19238 | 0.68 | 0.706899 |
Target: 5'- --aUACGGgcuggaCGUGACGAACaugcacgcauccgGCGACCu -3' miRNA: 3'- gaaGUGCCa-----GCACUGCUUG-------------CGCUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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