Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26028 | 5' | -58.5 | NC_005342.2 | + | 32542 | 0.66 | 0.545249 |
Target: 5'- aGGAACGccacgugcaacaugaGCG-CGGCCUGCuCGaGUCGGu -3' miRNA: 3'- gUCUUGC---------------CGCgGUUGGACG-GC-CAGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 10011 | 0.66 | 0.541048 |
Target: 5'- -cGAACGGCGCgGcgcGCUUGCuCGacUCGGg -3' miRNA: 3'- guCUUGCCGCGgU---UGGACG-GCc-AGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 44775 | 0.66 | 0.530595 |
Target: 5'- -cGGGCGGCGC--GCCggGCCGGcucacCGGc -3' miRNA: 3'- guCUUGCCGCGguUGGa-CGGCCa----GCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 17821 | 0.66 | 0.529554 |
Target: 5'- gCAGucgcuACGGCGCgCGAauucgguCCggcGCCGGUCGc -3' miRNA: 3'- -GUCu----UGCCGCG-GUU-------GGa--CGGCCAGCc -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 9451 | 0.66 | 0.520216 |
Target: 5'- ---cGCGGCGCC--CCaUGCCGGacauuUCGGc -3' miRNA: 3'- gucuUGCCGCGGuuGG-ACGGCC-----AGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 10567 | 0.66 | 0.520216 |
Target: 5'- -----gGGCcgGCCGACgaGCgGGUCGGg -3' miRNA: 3'- gucuugCCG--CGGUUGgaCGgCCAGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 1616 | 0.66 | 0.520216 |
Target: 5'- --cGGCGGCGUagcccugaauGCgCUGCgCGGUCGGa -3' miRNA: 3'- gucUUGCCGCGgu--------UG-GACG-GCCAGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 23147 | 0.66 | 0.520216 |
Target: 5'- -cGAuguUGGUGCCAucGCCcgucacGUCGGUCGGu -3' miRNA: 3'- guCUu--GCCGCGGU--UGGa-----CGGCCAGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 29636 | 0.66 | 0.514028 |
Target: 5'- uGGAACGGCGUgAggaucgugcgcacgcGCgUGCCGG-CGa -3' miRNA: 3'- gUCUUGCCGCGgU---------------UGgACGGCCaGCc -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 30214 | 0.66 | 0.499707 |
Target: 5'- -cGAGCGuGCgGCCGACCaggcUGCCGGcCa- -3' miRNA: 3'- guCUUGC-CG-CGGUUGG----ACGGCCaGcc -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 27277 | 0.66 | 0.499707 |
Target: 5'- -uGAACGGuCGCC-ACgaGCCGG-CGa -3' miRNA: 3'- guCUUGCC-GCGGuUGgaCGGCCaGCc -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 40864 | 0.67 | 0.489587 |
Target: 5'- uGGAACaguuuGCGCugcgauugCAugCUGCCGGUCuGGa -3' miRNA: 3'- gUCUUGc----CGCG--------GUugGACGGCCAG-CC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 4039 | 0.67 | 0.489587 |
Target: 5'- aCAGGuCGGCGgUcGCCaUGCCGGgcagcCGGa -3' miRNA: 3'- -GUCUuGCCGCgGuUGG-ACGGCCa----GCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 28478 | 0.67 | 0.489587 |
Target: 5'- -cGAGCGccGCGUCGACCgcGCgGGUCGcGg -3' miRNA: 3'- guCUUGC--CGCGGUUGGa-CGgCCAGC-C- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 45292 | 0.67 | 0.479563 |
Target: 5'- ---cAUGGCGCuCGACCaGCCcGGUCGc -3' miRNA: 3'- gucuUGCCGCG-GUUGGaCGG-CCAGCc -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 4946 | 0.67 | 0.440528 |
Target: 5'- uCGGcACGaGCGCaUAGCCgGCCGGgaUCGGc -3' miRNA: 3'- -GUCuUGC-CGCG-GUUGGaCGGCC--AGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 17944 | 0.67 | 0.440528 |
Target: 5'- gCAGGugcgACGGCgGCCAGCC-GCC-GUCGa -3' miRNA: 3'- -GUCU----UGCCG-CGGUUGGaCGGcCAGCc -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 8622 | 0.67 | 0.440528 |
Target: 5'- uCGGcGCGGCGcCCGGCUUGCuCGugacggugccGUCGGc -3' miRNA: 3'- -GUCuUGCCGC-GGUUGGACG-GC----------CAGCC- -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 29740 | 0.68 | 0.431053 |
Target: 5'- uCGGGAuCGGacaucaggaaGUCGAUCUGCUGGUCGa -3' miRNA: 3'- -GUCUU-GCCg---------CGGUUGGACGGCCAGCc -5' |
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26028 | 5' | -58.5 | NC_005342.2 | + | 31680 | 0.68 | 0.421699 |
Target: 5'- -uGAACa-CGCCGAagccgcCCUGCuCGGUCGGa -3' miRNA: 3'- guCUUGccGCGGUU------GGACG-GCCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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