Results 1 - 20 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26253 | 3' | -52.1 | NC_005345.2 | + | 24127 | 0.66 | 0.872219 |
Target: 5'- cCGCggUgaCCgaGGCGCagaccgauaacACAUGGAUCCc -3' miRNA: 3'- cGUGuuGaaGG--CCGCG-----------UGUACCUAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 6876 | 0.66 | 0.864014 |
Target: 5'- cGC-CGAUg-CCGcuGCGCAUGUGGAUCg -3' miRNA: 3'- -CGuGUUGaaGGC--CGCGUGUACCUAGg -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 1184 | 0.66 | 0.861503 |
Target: 5'- cGCACAcgggcgGCUcggcagUCCGGCccgcgggccacgccGCGCGguacGGGUCCc -3' miRNA: 3'- -CGUGU------UGA------AGGCCG--------------CGUGUa---CCUAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 37032 | 0.66 | 0.855555 |
Target: 5'- -aGCGGCacCgGGCGaGCcgGGAUCCg -3' miRNA: 3'- cgUGUUGaaGgCCGCgUGuaCCUAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 38564 | 0.66 | 0.855555 |
Target: 5'- cGCGCccaccGGCa--CGGCGUACGUGGcgCUc -3' miRNA: 3'- -CGUG-----UUGaagGCCGCGUGUACCuaGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 45528 | 0.66 | 0.855555 |
Target: 5'- gGCACAcCUg-CGGCGCGggcCGUGGggUCa -3' miRNA: 3'- -CGUGUuGAagGCCGCGU---GUACCuaGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 5075 | 0.66 | 0.846849 |
Target: 5'- aGCuGCAGCccaUCGGCGCuCGgcGGGUCCa -3' miRNA: 3'- -CG-UGUUGaa-GGCCGCGuGUa-CCUAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 42786 | 0.66 | 0.846849 |
Target: 5'- gGC-CGACUguagCCGGUGCGgAUGcGG-CCa -3' miRNA: 3'- -CGuGUUGAa---GGCCGCGUgUAC-CUaGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 22302 | 0.66 | 0.846849 |
Target: 5'- gGCACGuugcccGCgcUCGGCGCGCcgGGugCCg -3' miRNA: 3'- -CGUGU------UGaaGGCCGCGUGuaCCuaGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 11164 | 0.66 | 0.846849 |
Target: 5'- uGUACGGCggcaccCCGGCGCA----GGUCCg -3' miRNA: 3'- -CGUGUUGaa----GGCCGCGUguacCUAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 47990 | 0.66 | 0.837906 |
Target: 5'- cGCGCAcGCUccccUCgCGGCGCG-GUGGuGUCCc -3' miRNA: 3'- -CGUGU-UGA----AG-GCCGCGUgUACC-UAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 48040 | 0.66 | 0.836999 |
Target: 5'- aCGCGGCUgugcucgUCCGGCGCGCAccGcUCg -3' miRNA: 3'- cGUGUUGA-------AGGCCGCGUGUacCuAGg -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 22623 | 0.66 | 0.828735 |
Target: 5'- uGCGguGgUUCCGGCGCucgugccggguGCAagGGGUCg -3' miRNA: 3'- -CGUguUgAAGGCCGCG-----------UGUa-CCUAGg -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 30893 | 0.67 | 0.819346 |
Target: 5'- gGCgACGGCgcggCCGGCgagcaGCACcgGGA-CCa -3' miRNA: 3'- -CG-UGUUGaa--GGCCG-----CGUGuaCCUaGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 5207 | 0.67 | 0.80975 |
Target: 5'- gGCACGGCcggcCCGGCGCuACAcgccgucgGGGUgCCc -3' miRNA: 3'- -CGUGUUGaa--GGCCGCG-UGUa-------CCUA-GG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 42973 | 0.67 | 0.80975 |
Target: 5'- cGCACGAa--CCGGgcCGgGCGUGuGGUCCu -3' miRNA: 3'- -CGUGUUgaaGGCC--GCgUGUAC-CUAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 2787 | 0.67 | 0.799957 |
Target: 5'- gGUACGGCUUgUCGGCGCcgGCGUGcgcucGAUCg -3' miRNA: 3'- -CGUGUUGAA-GGCCGCG--UGUAC-----CUAGg -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 37948 | 0.67 | 0.799957 |
Target: 5'- cGC-CGACggugcCCGaGCGgAUcgGGAUCCg -3' miRNA: 3'- -CGuGUUGaa---GGC-CGCgUGuaCCUAGG- -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 43514 | 0.67 | 0.799957 |
Target: 5'- uCGCGGCgaacCCGGCGCucGCGgagcGGAUCg -3' miRNA: 3'- cGUGUUGaa--GGCCGCG--UGUa---CCUAGg -5' |
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26253 | 3' | -52.1 | NC_005345.2 | + | 40262 | 0.67 | 0.789979 |
Target: 5'- aCGgGGCg-CCGGCGgGCAUGaGUCCg -3' miRNA: 3'- cGUgUUGaaGGCCGCgUGUACcUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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