Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26269 | 3' | -56.7 | NC_005345.2 | + | 2828 | 0.66 | 0.612597 |
Target: 5'- cCAGCgCCgcgaGGCCGACA--GCGUGCg-- -3' miRNA: 3'- aGUUG-GG----CCGGCUGUacCGCACGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 47824 | 0.66 | 0.612597 |
Target: 5'- gCGACCCGGCCGGCAaccagaaGCagaaaUGCUUu -3' miRNA: 3'- aGUUGGGCCGGCUGUac-----CGc----ACGAAc -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 3947 | 0.66 | 0.612597 |
Target: 5'- --cGCCgCGGCCGGCAgccucucgGGCG-GCa-- -3' miRNA: 3'- aguUGG-GCCGGCUGUa-------CCGCaCGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 1892 | 0.66 | 0.612597 |
Target: 5'- -aGACCCgcgaggcaGGCCGACGguuguacgGGCGUcggcuacuGCUUGu -3' miRNA: 3'- agUUGGG--------CCGGCUGUa-------CCGCA--------CGAAC- -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 7277 | 0.66 | 0.601587 |
Target: 5'- aCAGCCCGGaCGAUgacGGUGUGUa-- -3' miRNA: 3'- aGUUGGGCCgGCUGua-CCGCACGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 14572 | 0.66 | 0.601587 |
Target: 5'- -gGGCCCGGCCGugucggcacuacACAaGGCGgagcgGCUc- -3' miRNA: 3'- agUUGGGCCGGC------------UGUaCCGCa----CGAac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 769 | 0.66 | 0.590604 |
Target: 5'- aUAGcCCCGGCCGGCGgcgGGgGaUGCc-- -3' miRNA: 3'- aGUU-GGGCCGGCUGUa--CCgC-ACGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 11191 | 0.66 | 0.590604 |
Target: 5'- gCAGCCUGGUCGACGccGGCcUGUg-- -3' miRNA: 3'- aGUUGGGCCGGCUGUa-CCGcACGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 35338 | 0.66 | 0.579656 |
Target: 5'- aUCGGCgCGGCCGGC---GCGUGgaUGu -3' miRNA: 3'- -AGUUGgGCCGGCUGuacCGCACgaAC- -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 21329 | 0.66 | 0.579656 |
Target: 5'- aCAGCUCGGUCGGguccucgccCGUGcGCG-GCUUGc -3' miRNA: 3'- aGUUGGGCCGGCU---------GUAC-CGCaCGAAC- -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 16861 | 0.66 | 0.568751 |
Target: 5'- gCAGCUgcaggcgcgguaCGGCCGAgCGUGGCG-GCg-- -3' miRNA: 3'- aGUUGG------------GCCGGCU-GUACCGCaCGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 3854 | 0.67 | 0.547105 |
Target: 5'- -gGACCCGGCUGAUc-GGCGUcgGCg-- -3' miRNA: 3'- agUUGGGCCGGCUGuaCCGCA--CGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 32988 | 0.67 | 0.53638 |
Target: 5'- cUCGGCgcaggCGGCCGGgGUGGCGgggGCg-- -3' miRNA: 3'- -AGUUGg----GCCGGCUgUACCGCa--CGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 14690 | 0.67 | 0.525728 |
Target: 5'- cCGGgCCGGCCGugccCGUGGUGUgGCg-- -3' miRNA: 3'- aGUUgGGCCGGCu---GUACCGCA-CGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 9325 | 0.67 | 0.522548 |
Target: 5'- gCGACCCGGgCGgcgaccagugcgacGCAUGGUG-GCUg- -3' miRNA: 3'- aGUUGGGCCgGC--------------UGUACCGCaCGAac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 42853 | 0.67 | 0.515157 |
Target: 5'- -gAACCCGGCCGGgAUgaaGGCG-GCc-- -3' miRNA: 3'- agUUGGGCCGGCUgUA---CCGCaCGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 6939 | 0.67 | 0.515157 |
Target: 5'- aCuuCCCGGUCGuCAUGGUgGUGCc-- -3' miRNA: 3'- aGuuGGGCCGGCuGUACCG-CACGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 23611 | 0.67 | 0.504673 |
Target: 5'- gCGACgCCGGCgGACcgGGCacgGCUg- -3' miRNA: 3'- aGUUG-GGCCGgCUGuaCCGca-CGAac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 6177 | 0.67 | 0.504673 |
Target: 5'- aCAcGCCCGGCCcgguuCGUGcGCGUGCc-- -3' miRNA: 3'- aGU-UGGGCCGGcu---GUAC-CGCACGaac -5' |
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26269 | 3' | -56.7 | NC_005345.2 | + | 41543 | 0.68 | 0.494282 |
Target: 5'- gCGACCUGGUCGACGacGuCGUGCg-- -3' miRNA: 3'- aGUUGGGCCGGCUGUacC-GCACGaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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