Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2636 | 3' | -61.8 | NC_001491.2 | + | 31507 | 0.66 | 0.654172 |
Target: 5'- cCGCCACCGGCCaCCc---GCCA-ACCu -3' miRNA: 3'- aGCGGUGGUCGG-GGagacCGGUcUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 32512 | 0.66 | 0.654172 |
Target: 5'- gCGuCCGCgaGGCCaaUCUGGCCAaGACg -3' miRNA: 3'- aGC-GGUGg-UCGGggAGACCGGU-CUGg -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 39442 | 0.66 | 0.650228 |
Target: 5'- -aGCCGCgGGCCCCagucgccugggugCUGGCCc--CCg -3' miRNA: 3'- agCGGUGgUCGGGGa------------GACCGGucuGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 140352 | 0.66 | 0.648255 |
Target: 5'- cCGCCguuaucggucacagcACCGGCCgCUCcGGUuuGACCu -3' miRNA: 3'- aGCGG---------------UGGUCGGgGAGaCCGguCUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 58533 | 0.66 | 0.644309 |
Target: 5'- aUCGCUAgCAcggcGUCCCUgacgCUGGCCAGGg- -3' miRNA: 3'- -AGCGGUgGU----CGGGGA----GACCGGUCUgg -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 32577 | 0.66 | 0.644309 |
Target: 5'- cCGCCggGCCGGagCCCUCaccGGCUccccaaGGACCa -3' miRNA: 3'- aGCGG--UGGUCg-GGGAGa--CCGG------UCUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 132986 | 0.66 | 0.634437 |
Target: 5'- aUUGCCACCAGUUCUUCUuugguguaGCCuGGACUc -3' miRNA: 3'- -AGCGGUGGUCGGGGAGAc-------CGG-UCUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 56667 | 0.66 | 0.634437 |
Target: 5'- aUGCCAugguuuccguCCAgGCCCCgaaUCUGGCC--ACCg -3' miRNA: 3'- aGCGGU----------GGU-CGGGG---AGACCGGucUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 2585 | 0.66 | 0.634437 |
Target: 5'- cCGCCACCAGCUCggggGGacagUAGGCCg -3' miRNA: 3'- aGCGGUGGUCGGGgagaCCg---GUCUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 98961 | 0.66 | 0.614694 |
Target: 5'- aCGCCgGCCuGGUCCCcCUGGgCGGcuGCCg -3' miRNA: 3'- aGCGG-UGG-UCGGGGaGACCgGUC--UGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 64509 | 0.67 | 0.608779 |
Target: 5'- gCGCCGCCAcacgucucuggggguGUCuCCUCcGGCCAcGGCg -3' miRNA: 3'- aGCGGUGGU---------------CGG-GGAGaCCGGU-CUGg -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 84028 | 0.67 | 0.604838 |
Target: 5'- -gGCgGCUggcGCCCCUCUGGUUucaggGGACUa -3' miRNA: 3'- agCGgUGGu--CGGGGAGACCGG-----UCUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 141195 | 0.67 | 0.595001 |
Target: 5'- cUCGCUgGCUGGCCgCggCUGGauaCAGACCa -3' miRNA: 3'- -AGCGG-UGGUCGGgGa-GACCg--GUCUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 11488 | 0.67 | 0.595001 |
Target: 5'- -gGCUGCCuGGCCCCUCUggggugggGGUCAGGg- -3' miRNA: 3'- agCGGUGG-UCGGGGAGA--------CCGGUCUgg -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 114238 | 0.67 | 0.585189 |
Target: 5'- aUUGCCAac--CCCCUCUgGGCCcuGGCCa -3' miRNA: 3'- -AGCGGUggucGGGGAGA-CCGGu-CUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 34406 | 0.67 | 0.585189 |
Target: 5'- -aGCCGgCGGCCCa---GGCCGGAgCCu -3' miRNA: 3'- agCGGUgGUCGGGgagaCCGGUCU-GG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 90540 | 0.67 | 0.585189 |
Target: 5'- aUCGUgGCUcugaaaaaCCCCUCUGGCCcaGCCa -3' miRNA: 3'- -AGCGgUGGuc------GGGGAGACCGGucUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 4681 | 0.67 | 0.579317 |
Target: 5'- -aGCCAUCcccgcgggcgguucgGGCCUCUCcagcgucuUGGCCAGAUUg -3' miRNA: 3'- agCGGUGG---------------UCGGGGAG--------ACCGGUCUGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 11913 | 0.67 | 0.575409 |
Target: 5'- -gGCCACCAcCCCCUCgaccggcacgcGGCaCGGcACCu -3' miRNA: 3'- agCGGUGGUcGGGGAGa----------CCG-GUC-UGG- -5' |
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2636 | 3' | -61.8 | NC_001491.2 | + | 8634 | 0.67 | 0.565666 |
Target: 5'- aUCcCCGCgGGCCCUgcgcccguacCUGGCCcggGGGCCa -3' miRNA: 3'- -AGcGGUGgUCGGGGa---------GACCGG---UCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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