Results 1 - 20 of 115 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26445 | 3' | -65.8 | NC_005357.1 | + | 13856 | 0.66 | 0.205187 |
Target: 5'- cUGCCGGUggccgacgugcGCGCCgcgauGGCCgGCGGCc-- -3' miRNA: 3'- aGCGGCCG-----------UGCGG-----CCGGgUGCCGcuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 27247 | 0.66 | 0.205187 |
Target: 5'- -aGCCGGCGCcCCaGGCUCuuGGcCGAGg -3' miRNA: 3'- agCGGCCGUGcGG-CCGGGugCC-GCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 14341 | 0.66 | 0.205187 |
Target: 5'- cUGCCgGGCcUGCUGGUUCGCGGUGu- -3' miRNA: 3'- aGCGG-CCGuGCGGCCGGGUGCCGCuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 8225 | 0.66 | 0.205187 |
Target: 5'- uUCGU--GCGCgGCCGuGCCCucguCGGCGAAg -3' miRNA: 3'- -AGCGgcCGUG-CGGC-CGGGu---GCCGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 10723 | 0.66 | 0.200019 |
Target: 5'- gCGCgCGGCGgGCUGGUCa--GGCGGu -3' miRNA: 3'- aGCG-GCCGUgCGGCCGGgugCCGCUu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 19631 | 0.66 | 0.200019 |
Target: 5'- -aGuCCGGCACcaugcugaCCGGCCCGCagGGCGu- -3' miRNA: 3'- agC-GGCCGUGc-------GGCCGGGUG--CCGCuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 7784 | 0.66 | 0.200019 |
Target: 5'- gCGCCGGCGuccacccuuCGCC-GCCCAgcaGGCGc- -3' miRNA: 3'- aGCGGCCGU---------GCGGcCGGGUg--CCGCuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 38419 | 0.66 | 0.194964 |
Target: 5'- aCGCUGGUG-GCCGGCgcguUUugGGCGGAc -3' miRNA: 3'- aGCGGCCGUgCGGCCG----GGugCCGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 30398 | 0.66 | 0.194964 |
Target: 5'- aCGCUgGGCAagaccuCGCCGGCC-ACGGCc-- -3' miRNA: 3'- aGCGG-CCGU------GCGGCCGGgUGCCGcuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 8207 | 0.66 | 0.194964 |
Target: 5'- gCGCaCGGCcucguagucGCGCUGGUCgGCGGCc-- -3' miRNA: 3'- aGCG-GCCG---------UGCGGCCGGgUGCCGcuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 34739 | 0.66 | 0.193966 |
Target: 5'- -aGCCGGC-CGCCGaggucaaGCCCGacgaccuggccccCGGUGAGa -3' miRNA: 3'- agCGGCCGuGCGGC-------CGGGU-------------GCCGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 33470 | 0.66 | 0.190021 |
Target: 5'- cCGCUGGCuauCGCggcaCGGCCacCGCuGGCGAAu -3' miRNA: 3'- aGCGGCCGu--GCG----GCCGG--GUG-CCGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 4051 | 0.66 | 0.190021 |
Target: 5'- gCGagCGGCAgGCCagcGGCCCAgGGgGGAu -3' miRNA: 3'- aGCg-GCCGUgCGG---CCGGGUgCCgCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 33422 | 0.66 | 0.190021 |
Target: 5'- cCGCUGGCuauCGCggcaCGGCCacCGCuGGCGAAu -3' miRNA: 3'- aGCGGCCGu--GCG----GCCGG--GUG-CCGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 25684 | 0.66 | 0.190021 |
Target: 5'- -aGCCGGCggauuucggcaACGCUGcucauaccaGcCCCGCGGCGGGg -3' miRNA: 3'- agCGGCCG-----------UGCGGC---------C-GGGUGCCGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 11835 | 0.66 | 0.190021 |
Target: 5'- -aGaCCGGcCGCGCCGcgguuGgCCugGGCGAAc -3' miRNA: 3'- agC-GGCC-GUGCGGC-----CgGGugCCGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 6244 | 0.66 | 0.190021 |
Target: 5'- -gGCCuGCACGCCGGUa-ACGGUGc- -3' miRNA: 3'- agCGGcCGUGCGGCCGggUGCCGCuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 6377 | 0.66 | 0.185667 |
Target: 5'- aCGUCGGCAaucaggcgcaggccgGCCGGCCCgguguagucGCGGCc-- -3' miRNA: 3'- aGCGGCCGUg--------------CGGCCGGG---------UGCCGcuu -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 31024 | 0.66 | 0.185189 |
Target: 5'- cUUGCCGGCcgaggcaaucGCGCgGGCCaACGugcGCGAAg -3' miRNA: 3'- -AGCGGCCG----------UGCGgCCGGgUGC---CGCUU- -5' |
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26445 | 3' | -65.8 | NC_005357.1 | + | 33427 | 0.66 | 0.185189 |
Target: 5'- cCGCCuGCugGaaGcGCCCccgcGCGGCGAGg -3' miRNA: 3'- aGCGGcCGugCggC-CGGG----UGCCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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